Western Blot (WB)
(Osteoprotegerin (OPG) mAb tested by Western blot. Osteoprotegerin (OPG) mAb detection by Western blot. The analysis was performed using Daudi nuclear extract and Osteoprotegerin (OPG) mAb at a 2 ug/ml dilution. The immunoreactivity was detected by enhanced chemiluminescence.)
Mouse anti-Human Osteoprotegerin Monoclonal Antibody | anti-TNFRSF11B antibody
Osteoprotegerin (OPG) antibody (mAb)(Clone 98A1071)
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Western Blot (WB)
(Osteoprotegerin (OPG) mAb tested by Western blot. Osteoprotegerin (OPG) mAb detection by Western blot. The analysis was performed using Daudi nuclear extract and Osteoprotegerin (OPG) mAb at a 2 ug/ml dilution. The immunoreactivity was detected by enhanced chemiluminescence.)
Western Blot (WB)
(Osteoprotegerin (OPG) mAb tested by Western blot. Osteoprotegerin (OPG) mAb detection by Western blot. The analysis was performed using Daudi nuclear extract and Osteoprotegerin (OPG) mAb at a 2 ug/ml dilution. The immunoreactivity was detected by enhanced chemiluminescence.)
Background: Osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) is a cytokine that can inhibit the production of osteoclasts. It is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a basic glycoprotein comprising 401 amino acid residues arranged into 7 structural domains. It is found as either a 60 kDa monomer or 120 kDa dimer linked by disulfide bonds. Osteoprotegerin inhibits the differentiation of osteoclast precursors into osteoclasts and also regulates the resorption of osteoclasts in vitro and in vivo. Osteoprotegerin is a RANK homolog, and works by binding to RANKL ligand on osteoblast/stromal cells, thus blocking the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
TNFRSF11B: Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD); also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal.
Protein type: Secreted; Inhibitor; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8q24
Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region
Molecular Function: cytokine activity; receptor activity
Biological Process: response to drug; response to magnesium ion; extracellular matrix organization and biogenesis; negative regulation of odontogenesis of dentine-containing teeth; response to estrogen stimulus; apoptosis; response to arsenic; signal transduction; negative regulation of bone resorption; skeletal development; response to nutrient
Disease: Paget Disease, Juvenile
Research Articles on TNFRSF11B
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Product Notes
The TNFRSF11B tnfrsf11b (Catalog #AAA388693) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Osteoprotegerin (OPG) antibody (mAb)(Clone 98A1071) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Osteoprotegerin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the TNFRSF11B tnfrsf11b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteoprotegerin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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