Rabbit anti-Human, Mouse Granzyme B Polyclonal Antibody | anti-GZMB antibody

Granzyme B (C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte Proteinase 2, Lymphocyte Protease, Fragmentin-2, Granzyme-2, Human Lymphocyte Protein, HLP, SECT, T-cell Serine Protease 1-3E, GZMB, CGL1, CSPB, CTLA1, GRB) (HRP)

Cat. Num. AAA6380213

• Gene Names: GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Granzyme B; Polyclonal Antibody; Granzyme B (C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte Proteinase 2; Lymphocyte Protease; Fragmentin-2; Granzyme-2; Human Lymphocyte Protein; HLP; SECT; T-cell Serine Protease 1-3E; GZMB; CGL1; CSPB; CTLA1; GRB) (HRP); anti-GZMB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GZMB. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GZMB antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human GZMB, aa1-247 (AAH30195.1).
• Immunogen Sequence: MQPILLLLAFLLLPRADAGEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIQDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRH
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GZMB rabbit polyclonal antibody. Western Blot analysis of GZMB expression in human liver.)

Western Blot (WB)

(GZMB rabbit polyclonal antibody. Western Blot analysis of GZMB expression in mouse kidney.)

Western Blot (WB)

(Western Blot analysis of GZMB expression in transfected 293T cell line by GZMB polyclonal antibody. Lane 1: GZMB transfected lysate (27.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-GZMB antibody

Granzyme A and granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes that are stored within cytolytic granules and released by effector cells during degradation. Granzyme B should be useful for localization of granzyme B-containing lytic granules and characterization of activated CTLs or NK cells.

Product Categories/Family for anti-GZMB antibody

Antibodies; Apoptosis

References

1. Granzyme B is a novel interleukin-18 converting enzyme. Omoto Y, Yamanaka K, Tokime K, Kitano S, Kakeda M, Akeda T, Kurokawa I, Gabazza EC, Tsutsui H, Katayama N, Yamanishi K, Nakanishi K, Mizutani H.J Dermatol Sci. 2010 Jun 17.

NCBI and Uniprot Product Information

• NCBI GI #: 20988817
• NCBI GeneID: 3002
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• Molecular Weight: 27,716 Da
• NCBI Official Full Name: Homo sapiens granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), mRNA
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• UniProt Protein Name: Granzyme B
• Protein Family: Granzyme
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
• UniProt Entry Name: GRAB_HUMAN

NCBI Description

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.79; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; immunological synapse; nucleus; cytosol

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; protein processing; immune response; proteolysis