Immunohistochemistry (IHC)
(Immunochemical staining of human RNF128 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human GRAIL/RNF128 Polyclonal Antibody | anti-RNF128 antibody
Anti-GRAIL/RNF128 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
GRAIL/RNF128; Polyclonal Antibody; Anti-GRAIL/RNF128 Antibody; Rabbit Polyclonal; RNF128 Antibody; Rabbit PAb; Antigen Affinity Purified; ring finger protein 128; E3 ubiquitin protein ligase; Anti-GRAIL Antibody; anti-RNF128 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RNF128
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RNF128 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human RNF128 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RNF128 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human RNF128 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human RNF128 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-RNF128 antibody
GRAIL (gene related to anergy in lymphocytes, also known as RNF128), a ubiquitin-protein ligase (E3), utilizes a unique single transmembrane protein with a split-function motif and is an important gatekeeper of T-cell unresponsiveness. RNF128 directly interacted with TBK1 through its protease-associated (PA) domain and catalyzed the K63-linked polyubiquitination of TBK1, which led to TBK1 activation, IRF3 activation, and IFN-beta production. Deficiency of RNF128 expression attenuated IRF3 activation, IFN-beta production, and innate antiviral immune responses to RNA and DNA viruses, in vitro and in vivo. RING finger protein 128 (RNF128) was shown to be involved in p53-induced apoptosis, forming a negative feedback loop. Downregulation of RNF128 is correlated with cancer invasiveness and metastasis as well as reduces survival in patients with upper urinary tract UC (UTUC) and urinary bladder UC (UBUC).
References
Whiting CC, et al. (2011) Grail: A unique mediator of cd4 t-lymphocyte unresponsiveness. Febs j 278 (1): 47-58.Song G, et al. (2016) E3 ubiquitin ligase rnf128 promotes innate antiviral immunity through k63-linked ubiquitination of tbk1. Nat Immunol 17 (12): 1342-1351.Lee YY, et al. (2016) Downregulation of rnf128 predicts progression and poor prognosis in patients with urothelial carcinoma of the upper tract and urinary bladder. J Cancer 7 (15): 2187-2196.
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Product Notes
The RNF128 (Catalog #AAA8108843) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRAIL/RNF128 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GRAIL/RNF128 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the RNF128 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRAIL/RNF128, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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