Goat GPX4 / MCSP Polyclonal Antibody | anti-GPX4 antibody

Goat Polyclonal to Human GPX4 / MCSP

Cat. Num. AAA242691

• Gene Names: GPX4; MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
• Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Bovine, Goat, Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GPX4 / MCSP; Polyclonal Antibody; Goat Polyclonal to Human GPX4 / MCSP; Anti-GPX4 / MCSP Antibody (C-Terminus) IHC-plus; GPX4; GPx-4; MCSP; SnPHGPx; Phospholipid hydroperoxidase; SnGPx; Glutathione peroxidase 4; GSHPx-4; PHGPx; Human GPX4; anti-GPX4 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Bovine, Goat, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GPX4. This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 197

• Applicable Applications for anti-GPX4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (2.5 ug/ml), WB (1:16000, 0.1 - 0.3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-EEPLVIEKDLPHY from the C-terminus of human GPX4 (NP_002076.2; NP_001034937.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Goat, Hamster, Bovine (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: GPX4 / MCSP antibody was raised against synthetic peptide C-EEPLVIEKDLPHY from the C-terminus of human GPX4 (NP_002076.2; NP_001034937.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Goat, Hamster, Bovine (92%); Marmoset, Mouse, Rat, Pig, Opossum, Guinea pig (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-GPX4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (0.1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-GPX4 antibody

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 75709200
• NCBI GeneID: 2879
• NCBI Accession #: NP_002076.2
• NCBI GenBank Nucleotide #: NM_002085.4
• UniProt Accession #: P36969; O43381; Q6PJ59; Q9UPK2
• Molecular Weight: 19,525 Da
• NCBI Official Full Name: phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A
• NCBI Official Synonym Full Names: glutathione peroxidase 4
• NCBI Official Symbol: GPX4
• NCBI Official Synonym Symbols: MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
• NCBI Protein Information: phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
• UniProt Protein Name: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• Protein Family: Probable glutathione peroxidase
• UniProt Gene Name: GPX4
• UniProt Synonym Gene Names: PHGPx; GPx-4; GSHPx-4
• UniProt Entry Name: GPX4_HUMAN

NCBI Description

This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Mitochondrial; Other Amino Acids Metabolism - glutathione; EC 1.11.1.12; Lipid Metabolism - arachidonic acid; Nuclear envelope; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; nucleus; cytosol

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity

Biological Process: glutathione metabolic process; lipoxygenase pathway; hydrogen peroxide catabolic process; phospholipid metabolic process; multicellular organismal development; regulation of inflammatory response; arachidonic acid metabolic process; response to oxidative stress; response to estradiol stimulus; aging