Rabbit anti-Human NQO1 Polyclonal Antibody | anti-NQO1 antibody

NQO1 Antibody

Cat. Num. AAA9414157

• Gene Names: NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: NQO1; Polyclonal Antibody; NQO1 Antibody; DHQU; DIA4; DTD; NMOR1; NMORI; anti-NQO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total NQO1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 274

• Applicable Applications for anti-NQO1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human NQO1.
• Target Name: NQO1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NQO1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human thyroid cancer using NQO1 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney using NQO1 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human rectal cancer using NQO1 antibody at dilution of 1:200 (200x lens).)

Related Product Information for anti-NQO1 antibody

NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that catalyzes the two-electron reduction of quinones and their derivatives (1,2). This enzyme protects cells against redox cycling and oxidative stress (1,3). The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues (1,2). Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development (1). Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turns leads to myeloid hyperplasia of bone marrow (2).

Product Categories/Family for anti-NQO1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4505415
• NCBI GeneID: 1728
• NCBI Accession #: NP_000894.1
• NCBI GenBank Nucleotide #: NM_000903.2
• UniProt Accession #: P15559; Q86UK1; B2R5Y9; B4DNM7; B7ZAD1
• Molecular Weight: 26,365 Da
• NCBI Official Full Name: NAD(P)H dehydrogenase
• NCBI Official Synonym Full Names: NAD(P)H dehydrogenase, quinone 1
• NCBI Official Symbol: NQO1
• NCBI Official Synonym Symbols: DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• NCBI Protein Information: NAD(P)H dehydrogenase [quinone] 1
• UniProt Protein Name: NAD(P)H dehydrogenase [quinone] 1
• Protein Family: NAD(P)H dehydrogenase
• UniProt Gene Name: NQO1
• UniProt Synonym Gene Names: DIA4; NMOR1; DTD; QR1
• UniProt Entry Name: NQO1_HUMAN

NCBI Description

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer's disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.6.5.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell soma; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; NAD(P)H dehydrogenase (quinone) activity; cytochrome-b5 reductase activity; superoxide dismutase activity

Biological Process: response to ethanol; removal of superoxide radicals; xenobiotic metabolic process; response to toxin; positive regulation of neuron apoptosis; negative regulation of catalytic activity; regulation of amino acid metabolic process; nitric oxide biosynthetic process; response to estradiol stimulus; response to nutrient; aging; synaptic transmission, cholinergic