Goat GPR183 Polyclonal Antibody | anti-GPR183 antibody

Goat anti-GPR183 Antibody

Cat. Num. AAA422829

• Gene Names: GPR183; EBI2
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Cow, Rabbit
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GPR183; Polyclonal Antibody; Goat anti-GPR183 Antibody; G protein-coupled receptor 183; EBI2; EBV-induced G-protein coupled receptor 2; Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor); G-protein coupled receptor 183; epstein-Barr virus-induced G-protein coupled recept; GPR183 antibody; G protein-coupled receptor 183 antibody; EBI2 antibody; EBV-induced G-protein coupled receptor 2 antibody; Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor) antibody; G-protein coupled receptor 183 antibody; epstein-Barr virus-induced G-protein coupled receptor 2 antibody; anti-GPR183 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Cow, Rabbit
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: NKIKRIEHAK
• Sequence Length: 361

• Applicable Applications for anti-GPR183 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Preliminary experiments gave an approx 60kDa band in lysates of cell line Daudi after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kDa according to NP_004942.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-NKIKRIEHAK, from the internal region of the protein sequence according to NP_004942.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 4826706
• NCBI GeneID: 1880
• NCBI Accession #: NP_004942.1
• NCBI GenBank Nucleotide #: NM_004951.4
• Molecular Weight: 41,224 Da
• NCBI Official Full Name: G-protein coupled receptor 183
• NCBI Official Synonym Full Names: G protein-coupled receptor 183
• NCBI Official Symbol: GPR183
• NCBI Official Synonym Symbols: EBI2
• NCBI Protein Information: G-protein coupled receptor 183
• UniProt Protein Name: G-protein coupled receptor 183
• Protein Family: G-protein coupled receptor
• UniProt Gene Name: GPR183
• UniProt Synonym Gene Names: EBI2; EBI2; EBV-induced G-protein coupled receptor 2
• UniProt Entry Name: GP183_HUMAN

NCBI Description

This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

GPR183: Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7- alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses. Signals constitutively through G(i)-alpha, but not throughG(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2). Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 13q32.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; oxysterol binding

Biological Process: adaptive immune response; B cell activation during immune response; G-protein coupled receptor protein signaling pathway; humoral immune response; immune response; mature B cell differentiation during immune response; positive regulation of B cell proliferation