Immunohistochemistry
Rabbit BDNF Polyclonal Antibody | anti-BDNF antibody
Rabbit anti BDNF Polyclonal
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of BDNF expression in paraffin embedded formalin fixed Rat brain tissue.
It was used as primary antibody, dilution 1:200.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Immunohistochemistry
Immunohistochemistry
• postsynaptic trpc1 function contributes to BDNF-induced synaptic potentiation at the developing neuromuscular junction .J. Neurosci., Oct 2011; 31: 14754 - 14762.
• levels of BDNF impact oligodendrocyte lineage cells following a cuprizone lesion .J. Neurosci., Oct 2011; 31: 14182 - 14190.
• spatial segregation of BDNF transcripts enables BDNF to differentially shape distinct dendritic compartments .PNAS, Oct 2011; 108: 16813 - 16818.
• brain-derived neurotrophic factor (BDNF) enhances gaba transport by modulating the trafficking of gaba transporter-1 (gat-1) from the plasma membrane of rat cortical astrocytes .J. Biol. Chem., Oct 2011; 10.1074/jbc.M111.232009.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]
Uniprot Description
CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Cell adhesion
Chromosomal Location of Human Ortholog: 6q23.1
Cellular Component: Golgi apparatus; extracellular space; proteinaceous extracellular matrix; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; extracellular region; plasma membrane; cell cortex; cytosol
Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; protein binding; growth factor activity; fibronectin binding
Biological Process: response to peptide hormone stimulus; epidermis development; response to anoxia; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of caspase activity; positive regulation of JNK cascade; cellular lipid metabolic process; response to estradiol stimulus; cell-cell signaling; positive regulation of stress fiber formation; positive regulation of cell proliferation; response to wounding; tissue homeostasis; response to glucose stimulus; regulation of cell growth; angiogenesis; cell differentiation; cell adhesion; cartilage condensation; positive regulation of cell activation; integrin-mediated signaling pathway; transcription initiation from RNA polymerase II promoter; cell migration; ossification; fibroblast growth factor receptor signaling pathway; organ senescence; response to mineralocorticoid stimulus; response to amino acid stimulus; gene expression; positive regulation of protein amino acid phosphorylation; regulation of chondrocyte differentiation; positive regulation of cell differentiation; lung development
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Product Notes
The BDNF ctgf (Catalog #AAA551100) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti BDNF Polyclonal reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BDNF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA). ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of BDNF expression in paraffin embedded formalin fixed Rat brain tissue. It was used as primary antibody, dilution 1:200. Researchers should empirically determine the suitability of the BDNF ctgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BDNF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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