Flow Cytometry (FC/FACS)
Rabbit anti-Mouse, Rat GP2 Polyclonal Antibody | anti-Gp2 antibody
Anti-GP2 Antibody
Gene Names
Gp2; AV060639; 2310037I18Rik
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
GP2; Polyclonal Antibody; Anti-GP2 Antibody; Pancreatic secretory granule membrane major glycoprotein GP2; Pancreatic zymogen granule membrane protein GP-2; Gp2; anti-Gp2 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
534
Applicable Applications for anti-Gp2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Rat)
FC/FACS: 1-3ug/1x106 cells (Rat)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Rat)
FC/FACS: 1-3ug/1x106 cells (Rat)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived mouse GP2 recombinant protein (Position: S35-R497).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Western Blot (WB)
(Figure 1. Western blot analysis of Gp2 using anti-Gp2 antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of Gp2 using anti-Gp2 antibody.)
Related Product Information for anti-Gp2 antibody
Description: Rabbit IgG polyclonal antibody for GP2 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Mouse; Rat.
Background: Pancreatic secretory granule membrane major glycoprotein GP2 is a protein that in humans is encoded by the GP2 gene. It is mapped to 7 F2; 7 63.88 cM. This gene encodes an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. The encoded protein binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). Alternative splicing results in multiple transcript variants.
Background: Pancreatic secretory granule membrane major glycoprotein GP2 is a protein that in humans is encoded by the GP2 gene. It is mapped to 7 F2; 7 63.88 cM. This gene encodes an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. The encoded protein binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). Alternative splicing results in multiple transcript variants.
References
1. Fukuoka, S.-I., Freedman, S. D., Scheele, G. A. A single gene encodes membrane-bound and free forms of GP-2, the major glycoprotein in pancreatic secretory (zymogen) granule membranes. Proc. Nat. Acad. Sci. 88: 2898-2902, 1991.
2. Fukuoka, S.-I., Freedman, S. D., Yu, H., Sukhatme, V. P., Scheele, G. A. GP-2/THP gene family encodes self-binding glycosylphosphatidylinositol-anchored proteins in apical secretory compartments of pancreas and kidney. Proc. Nat. Acad. Sci. 89: 1189-1193, 1992. Note: Erratum: Proc. Nat. Acad. Sci. 89: 3669 only, 1992.
3. Fukuoka, S.-I., Suzuki, M., Okabayashi, K., Takahashi, E. Assignment of pancreatic zymogen granule membrane protein GP2 (GP2) to human chromosome band 9q21.11 to q21.2 by in situ hybridization. Cytogenet. Cell Genet. 79: 231-232, 1997.
2. Fukuoka, S.-I., Freedman, S. D., Yu, H., Sukhatme, V. P., Scheele, G. A. GP-2/THP gene family encodes self-binding glycosylphosphatidylinositol-anchored proteins in apical secretory compartments of pancreas and kidney. Proc. Nat. Acad. Sci. 89: 1189-1193, 1992. Note: Erratum: Proc. Nat. Acad. Sci. 89: 3669 only, 1992.
3. Fukuoka, S.-I., Suzuki, M., Okabayashi, K., Takahashi, E. Assignment of pancreatic zymogen granule membrane protein GP2 (GP2) to human chromosome band 9q21.11 to q21.2 by in situ hybridization. Cytogenet. Cell Genet. 79: 231-232, 1997.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pancreatic secretory granule membrane major glycoprotein GP2
NCBI Official Synonym Full Names
glycoprotein 2 (zymogen granule membrane)
NCBI Official Symbol
Gp2
NCBI Official Synonym Symbols
AV060639; 2310037I18Rik
NCBI Protein Information
pancreatic secretory granule membrane major glycoprotein GP2
UniProt Protein Name
Pancreatic secretory granule membrane major glycoprotein GP2
Protein Family
UniProt Gene Name
Gp2
UniProt Entry Name
GP2_MOUSE
Research Articles on Gp2
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Product Notes
The Gp2 gp2 (Catalog #AAA1753140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GP2 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Rat) FC/FACS: 1-3ug/1x106 cells (Rat) Direct ELISA: 0.1-0.5ug/ml (Mouse) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Gp2 gp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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