Flow Cytometry (FC/FACS)
Rabbit PAR1/Thrombin Receptor/F2R Polyclonal Antibody | anti-F2R antibody
Anti-PAR1/Thrombin Receptor/F2R Antibody
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Western Blot (WB)
(Figure 1. Western blot analysis of F2R using anti-F2R antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of F2R using anti-F2R antibody.)
Background: Proteinase-activated receptor 1 (PAR1), also known as the coagulation factor II (thrombin) receptor, is a protein that in humans is encoded by the F2R gene. By fluorescence in situ hybridization, this gene is mapped to 5q13, confirming its presence as a single locus in the human genome. PAR1 is a G protein-coupled receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. The expression of PAR1 is both required and sufficient to promote growth and invasion of breast carcinoma cells in a xenograft mouse model.
2. Boire, A., Covic, L., Agarwal, A., Jacques, S., Sherifi, S., Kuliopulos, A. PAR1 is a matrix metalloprotease-1 receptor that promotes invasion and tumorigenesis of breast cancer cells. Cell 120: 303-131, 2005.
3. Chu, H.-W., Cheng, C.-W., Chou, W.-C., Hu, L.-Y., Wang, H.-W., Hsiung, C.-N., Hsu, H.-M., Wu, P.-E., Hou, M.-F., Shen, C.-Y., Yu, J.-C. A novel estrogen receptor-microRNA 190a-PAR-1-pathway regulates breast cancer progression, a finding initially suggested by genome-wide analysis of loci associated with lymph-node metastasis. Hum. Molec. Genet. 23: 355-367, 2014.
NCBI and Uniprot Product Information
NCBI Description
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
Uniprot Description
PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Cell development/differentiation
Chromosomal Location of Human Ortholog: 5q13
Cellular Component: Golgi apparatus; postsynaptic membrane; integral to plasma membrane; early endosome; extracellular region; plasma membrane; caveola; neuromuscular junction
Molecular Function: G-protein coupled receptor activity; protein binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; receptor binding; thrombin receptor activity
Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of blood coagulation; negative regulation of glomerular filtration; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; positive regulation of caspase activity; positive regulation of JAK-STAT cascade; response to lipopolysaccharide; negative regulation of cell proliferation; connective tissue replacement during inflammatory response; elevation of cytosolic calcium ion concentration; platelet dense granule organization and biogenesis; positive regulation of MAPKKK cascade; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of cell proliferation; response to wounding; negative regulation of neuron apoptosis; positive regulation of smooth muscle contraction; establishment of synaptic specificity at neuromuscular junction; inflammatory response; STAT protein nuclear translocation; caspase activation; platelet activation; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of Rho protein signal transduction; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; regulation of blood coagulation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of release of sequestered calcium ion into cytosol; regulation of sensory perception of pain; positive regulation of calcium ion transport; blood coagulation; positive regulation of cell migration
Research Articles on F2R
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Product Notes
The F2R f2r (Catalog #AAA1753076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PAR1/Thrombin Receptor/F2R Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAR1/Thrombin Receptor/F2R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Human, Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Human) FC/FACS: 1-3ug/1x106 cells (Human) Direct ELISA: 0.1-0.5ug/ml (Human) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the F2R f2r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAR1/Thrombin Receptor/F2R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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