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Flow Cytometry (FC/FACS)
Rabbit anti-Human VMAT1/SLC18A1 Polyclonal Antibody | anti-SLC18A1 antibody
Anti-VMAT1/SLC18A1 Antibody
Gene Names
SLC18A1; CGAT; VAT1; VMAT1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
VMAT1/SLC18A1; Polyclonal Antibody; Anti-VMAT1/SLC18A1 Antibody; Chromaffin granule amine transporter; Solute carrier family 18 member 1; Vesicular amine transporter 1; VAT1; SLC18A1; VMAT1; anti-SLC18A1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
525
Applicable Applications for anti-SLC18A1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Human)
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived human VMAT1/SLC18A1 recombinant protein (Position: M1-E510).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Western Blot (WB)
(Figure 1. Western blot analysis of SLC18A1 using anti-SLC18A1 antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of SLC18A1 using anti-SLC18A1 antibody.)
Related Product Information for anti-SLC18A1 antibody
Description: Rabbit IgG polyclonal antibody for VMAT1/SLC18A1 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human.
Background: Chromaffin granule amine transporter is a protein that in humans is encoded by the SLC18A1 gene. This gene is mapped to 8p21.3. The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles, using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine.
Background: Chromaffin granule amine transporter is a protein that in humans is encoded by the SLC18A1 gene. This gene is mapped to 8p21.3. The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles, using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine.
References
1. Liu, Y., Peter, D., Roghani, A., Schuldiner, S., Prive, G. G., Eisenberg, D., Brecha, N., Edwards, R. H. A cDNA that suppresses MPP+ toxicity encodes a vesicular amine transporter. Cell 70: 539-551, 1992.
2. Peter, D., Finn, J. P., Klisak, I., Liu, Y., Kojis, T., Heinzmann, C., Roghani, A., Sparkes, R. S., Edwards, R. H. Chromosomal localization of the human vesicular amine transporter genes. Genomics 18: 720-723, 1993.
3. Roghani, A., Welch, C., Xia, Y.-R., Liu, Y., Peter, D., Finn, J. P., Edwards, R. H., Lusis, A. J. Assignment of the mouse vesicular monoamine transporter genes, Slc18a1 and Slc18a2, to chromosomes 8 and 19 by linkage analysis. Mammalian Genome 7: 393-394, 1996.
2. Peter, D., Finn, J. P., Klisak, I., Liu, Y., Kojis, T., Heinzmann, C., Roghani, A., Sparkes, R. S., Edwards, R. H. Chromosomal localization of the human vesicular amine transporter genes. Genomics 18: 720-723, 1993.
3. Roghani, A., Welch, C., Xia, Y.-R., Liu, Y., Peter, D., Finn, J. P., Edwards, R. H., Lusis, A. J. Assignment of the mouse vesicular monoamine transporter genes, Slc18a1 and Slc18a2, to chromosomes 8 and 19 by linkage analysis. Mammalian Genome 7: 393-394, 1996.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Chromaffin granule amine transporter
NCBI Official Synonym Full Names
solute carrier family 18 member A1
NCBI Official Symbol
SLC18A1
NCBI Official Synonym Symbols
CGAT; VAT1; VMAT1
NCBI Protein Information
chromaffin granule amine transporter
UniProt Protein Name
Chromaffin granule amine transporter
Protein Family
UniProt Gene Name
SLC18A1
UniProt Synonym Gene Names
VAT1; VMAT1; VAT1
UniProt Entry Name
VMAT1_HUMAN
NCBI Description
The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles, using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (Peter et al., 1993 [PubMed 7905859]). See also SLC18A2 (MIM 193001).[supplied by OMIM, Mar 2008]
Uniprot Description
SLC18A1: Involved in the vesicular transport of biogenic amines. Belongs to the major facilitator superfamily. Vesicular transporter family.
Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter
Chromosomal Location of Human Ortholog: 8p21.3
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: monoamine transmembrane transporter activity; drug transporter activity
Biological Process: neurotransmitter transport; monoamine transport; multidrug transport; transmembrane transport
Research Articles on SLC18A1
Similar Products
Product Notes
The SLC18A1 slc18a1 (Catalog #AAA1753155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VMAT1/SLC18A1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VMAT1/SLC18A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Human) IHC-P (Embedded Section): 0.5-1ug/ml (Human) FC/FACS: 1-3ug/1x106 cells (Human) Direct ELISA: 0.1-0.5ug/ml (Human) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the SLC18A1 slc18a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VMAT1/SLC18A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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