Rabbit Cyclin B2/CCNB2 Polyclonal Antibody | anti-CCNB2 antibody

Anti-Cyclin B2/CCNB2 Antibody

Cat. Num. AAA1753014

• Gene Names: CCNB2; HsT17299
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Cyclin B2/CCNB2; Polyclonal Antibody; Anti-Cyclin B2/CCNB2 Antibody; G2/mitotic-specific cyclin-B2; CCNB2; anti-CCNB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 398

• Applicable Applications for anti-CCNB2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human, Mouse, Rat); IHC-P (Embedded Section): 0.5-1ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human Cyclin B2/CCNB2 recombinant protein (Position: K23-A391).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

(Figure 1. Western blot analysis of CCNB2 using anti-CCNB2 antibody.)

Related Product Information for anti-CCNB2 antibody

Description: Rabbit IgG polyclonal antibody for Cyclin B2/CCNB2 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human; Mouse; Rat.

Background: G2/mitotic-specific cyclin-B2 is a protein that in humans is encoded by the CCNB2 gene. This gene is mapped to 15q22.2. Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

References

1. Brandeis, M., Rosewell, I., Carrington, M., Crompton, T., Jacobs, M. A., Kirk, J., Gannon, J., Hunt, T. Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero. Proc. Nat. Acad. Sci. 95: 4344-4349, 1998.
2. Jackman, M., Firth, M., Pines, J. Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus. EMBO J. 14: 1646-1654, 1995.
3. Liu, J. H., Wei, S., Burnette, P. K., Gamero, A. M., Hutton, M., Djeu, J. Y. Functional association of TGF-beta receptor II with cyclin B. Oncogene 18: 269-275, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 5921731
• NCBI GeneID: 9133
• UniProt Accession #: O95067
• NCBI Official Full Name: G2/mitotic-specific cyclin-B2
• NCBI Official Synonym Full Names: cyclin B2
• NCBI Official Symbol: CCNB2
• NCBI Official Synonym Symbols: HsT17299
• NCBI Protein Information: G2/mitotic-specific cyclin-B2
• UniProt Protein Name: G2/mitotic-specific cyclin-B2
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: CCNB2
• UniProt Entry Name: CCNB2_HUMAN

NCBI Description

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; membrane; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth