Rabbit GNB1 Polyclonal Antibody | anti-GNB1 antibody

GNB1 Polyclonal Antibody

Cat. Num. AAA3011444

• Gene Names: GNB1; MRD42
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GNB1; Polyclonal Antibody; GNB1 Polyclonal Antibody; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; Transducin beta chain 1; anti-GNB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GNB1 polyclonal antibody detects endogenous levels of GNB1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 240

• Applicable Applications for anti-GNB1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:4000
• Immunogen: Recombinant full length Human GNB1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using GNB1 antibody at 1:1000 dilution.)

Related Product Information for anti-GNB1 antibody

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (i.e., a photon, pheromone, odorant, hormone or neurotransmitter), while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein a, b and g polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Evidence, however, has established an important regulatory role for the b and g subunits. The G protein b subunits are important regulators of G protein a subunits as well as of certain signal transduction receptors and effectors. In mammals, there are five different members of the b subunit family

NCBI and Uniprot Product Information

• NCBI GI #: 543173167
• NCBI GeneID: 2782
• NCBI Accession #: NP_001269467.1
• NCBI GenBank Nucleotide #: NP_001269467.1
• UniProt Accession #: P62873
• Molecular Weight: ~ 37kDa
• NCBI Official Full Name: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 isoform 2
• NCBI Official Synonym Full Names: G protein subunit beta 1
• NCBI Official Symbol: GNB1
• NCBI Official Synonym Symbols: MRD42
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Gene Name: GNB1

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family.

Protein type: G protein; G protein, heterotrimeric; G protein, heterotrimeric beta

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytosol; heterotrimeric G-protein complex; intracellular; lysosomal membrane; membrane; plasma membrane

Molecular Function: GTPase activity; GTPase binding; protein binding; protein complex binding

Biological Process: acetylcholine receptor signaling, muscarinic pathway; dopamine receptor, adenylate cyclase activating pathway; platelet activation; protein folding; Ras protein signal transduction; rhodopsin mediated signaling; signal transduction; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Leukemia, Acute Lymphoblastic; Mental Retardation, Autosomal Dominant 42