Rabbit anti-Human GNB1 Polyclonal Antibody | anti-GNB1 antibody

GNB1, NT (GNB1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1) (Biotin)

Cat. Num. AAA6302998

• Gene Names: GNB1; MRD42
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GNB1; Polyclonal Antibody; NT (GNB1; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; Transducin beta chain 1) (Biotin); Transducin beta chain 1; anti-GNB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GNB1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GNB1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GNB1 Antibody (N-term) in Jurkat cell line lysates (35ug/lane).GNB1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(GNB1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GNB1 antibody

GNB1 integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This protein uses alternative polyadenylation signals.

Product Categories/Family for anti-GNB1 antibody

Antibodies; G Proteins

References

Ahmed, S.M., et al. J. Biol. Chem. 285(9):6538-6551(2010). Gutman, O., et al. J. Biol. Chem. 285(6):3905-3915(2010). Knezevic, N., et al. J. Exp. Med. 206(12):2761-2777(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 543173167
• NCBI GeneID: 2782
• NCBI Accession #: NP_001269467.1
• NCBI GenBank Nucleotide #: NM_001282538.1
• UniProt Accession #: P62873; P04697; P04901; Q1RMY8; B1AJZ7
• Molecular Weight: 36,298 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 isoform 2
• NCBI Official Synonym Full Names: G protein subunit beta 1
• NCBI Official Symbol: GNB1
• NCBI Official Synonym Symbols: MRD42
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Gene Name: GNB1

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family.

Protein type: G protein; G protein, heterotrimeric; G protein, heterotrimeric beta

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytosol; heterotrimeric G-protein complex; intracellular; lysosomal membrane; membrane; plasma membrane

Molecular Function: GTPase activity; GTPase binding; protein binding; protein complex binding

Biological Process: acetylcholine receptor signaling, muscarinic pathway; dopamine receptor, adenylate cyclase activating pathway; platelet activation; protein folding; Ras protein signal transduction; rhodopsin mediated signaling; signal transduction; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Leukemia, Acute Lymphoblastic; Mental Retardation, Autosomal Dominant 42