Rabbit anti-Human, Mouse GNAT1 Polyclonal Antibody | anti-GNAT1 antibody

GNAT1 Antibody

Cat. Num. AAA7052001

• Gene Names: GNAT1; GBT1; GNATR; CSNB1G; CSNBAD3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: GNAT1; Polyclonal Antibody; GNAT1 Antibody; Guanine nucleotide-binding protein G(t) subunit alpha-1; Transducin alpha-1 chain; GNATR; anti-GNAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 350

• Applicable Applications for anti-GNAT1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human Guanine nucleotide-binding protein G(t) subunit alpha-1 protein (92-170AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse liver tissueAll lanes: GNAT1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 41 KDaObserved band size: 41 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7052001 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7052001 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-GNAT1 antibody

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

NCBI and Uniprot Product Information

• NCBI GI #: 22027520
• NCBI GeneID: 2779
• NCBI Accession #: NP_000163.2
• NCBI GenBank Nucleotide #: NM_000172.3
• UniProt Accession #: P11488; Q4VBN2
• Molecular Weight: 40,041 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(t) subunit alpha-1
• NCBI Official Synonym Full Names: G protein subunit alpha transducin 1
• NCBI Official Symbol: GNAT1
• NCBI Official Synonym Symbols: GBT1; GNATR; CSNB1G; CSNBAD3
• NCBI Protein Information: guanine nucleotide-binding protein G(t) subunit alpha-1
• UniProt Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-1
• UniProt Gene Name: GNAT1
• UniProt Synonym Gene Names: GNATR

NCBI Description

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]

Uniprot Description

G-alpha t1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3); also known as congenital stationary night blindness Nougaret type. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein; G protein, heterotrimeric; G protein, heterotrimeric alpha G((i/o/t/z))

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytosol; heterotrimeric G-protein complex; membrane; photoreceptor inner segment; photoreceptor outer segment; plasma membrane

Molecular Function: acyl binding; G-protein beta/gamma-subunit binding; G-protein-coupled receptor binding; GDP binding; GTP binding; GTPase activity; protein kinase binding; signal transducer activity

Biological Process: detection of light stimulus involved in visual perception; G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of cyclic-nucleotide phosphodiesterase activity; phototransduction, visible light; protein folding; regulation of rhodopsin mediated signaling; response to light stimulus; rhodopsin mediated signaling; sensory perception of umami taste; visual perception

Disease: Night Blindness, Congenital Stationary, Autosomal Dominant 3; Night Blindness, Congenital Stationary, Type 1g