Western Blot (WB)
((0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EDF1 Polyclonal Antibody | anti-EDF1 antibody
Goat anti-EDF1 Antibody
Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
EDF1: Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription, coactivator/corepressor; RNA-binding; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: cytoplasm; intracellular; nucleolus; nucleus; transcription factor TFIID complex
Molecular Function: calmodulin binding; histone acetyltransferase activity; methyltransferase activity; protein binding; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity
Biological Process: endothelial cell differentiation; multicellular organismal development; positive regulation of DNA binding; positive regulation of transcription, DNA-dependent; regulation of lipid metabolic process; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on EDF1
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Product Notes
The EDF1 edf1 (Catalog #AAA423373) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EDF1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's EDF1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10ug/ml. Western Blot: Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the EDF1 edf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NKQHSITKNT AKLDR. It is sometimes possible for the material contained within the vial of "EDF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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