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Western Blot (WB) (GNAS Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GNAS antibody detected the GNAS protein (arrow).)

Rabbit anti-Human GNAS Polyclonal Antibody | anti-GNAS antibody

GNAS, CT (GNAS, GNAS1, Neuroendocrine secretory protein 55, LHAL tetrapeptide, GPIPIRRH peptide) (PE)

Gene Names
GNAS; AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GNAS; Polyclonal Antibody; CT (GNAS; GNAS1; Neuroendocrine secretory protein 55; LHAL tetrapeptide; GPIPIRRH peptide) (PE); GPIPIRRH peptide; anti-GNAS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GNAS.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-GNAS antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide between 216-244aa from the C-terminal region of human GNAS conjugated to KLH.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GNAS Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GNAS antibody detected the GNAS protein (arrow).)

Western Blot (WB) (GNAS Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GNAS antibody detected the GNAS protein (arrow).)
Related Product Information for anti-GNAS antibody
This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein-Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Product Categories/Family for anti-GNAS antibody
References
1. Idziaszczyk, S., et al. (2010)Cancer Genet. Cytogenet. 202(1):67-69. 2. Bailey, S.D., et al. (2010)Diabetes Care 33(10):2250-2253. 3. Tominaga, E., et al. (2010)Gynecol. Oncol. 118(2):160-166. 4. Park, C.H., et al. (2010)Ann. Clin. Lab. Sci. 40(3):261-266. 5. Cross, D.S., et al. (2010). BMC Genet. 11, 51.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,029 Da
NCBI Official Full Name
protein NESP55 isoform NESP55
NCBI Official Synonym Full Names
GNAS complex locus
NCBI Official Symbol
GNAS
NCBI Official Synonym Symbols
AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45
NCBI Protein Information
protein ALEX; protein GNAS; protein NESP55; secretogranin VI; extra large alphas protein; neuroendocrine secretory protein; guanine nucleotide regulatory protein; alternative gene product encoded by XL-exon; adenylate cyclase-stimulating G alpha protein;
UniProt Protein Name
Neuroendocrine secretory protein 55
Protein Family
UniProt Gene Name
GNAS
UniProt Synonym Gene Names
GNAS1; NESP55
UniProt Entry Name
GNAS3_HUMAN

NCBI Description

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

Uniprot Description

G-alpha(s) iso7: a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein type: G protein, heterotrimeric alpha G(s); G protein, heterotrimeric; G protein; Membrane protein, peripheral; Oncoprotein; Vesicle

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: ruffle; transport vesicle; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; extracellular region; plasma membrane; heterotrimeric G-protein complex; trans-Golgi network membrane; intrinsic to membrane; nucleus; cytosol

Molecular Function: GTPase activity; corticotropin-releasing hormone receptor 1 binding; insulin-like growth factor receptor binding; ionotropic glutamate receptor binding; signal transducer activity; protein binding; GTP binding; mu-type opioid receptor binding; metal ion binding; beta-2 adrenergic receptor binding; G-protein beta/gamma-subunit binding; D1 dopamine receptor binding; adenylate cyclase activity

Biological Process: developmental growth; positive regulation of osteoclast differentiation; water transport; protein secretion; female pregnancy; G-protein signaling, adenylate cyclase activating pathway; embryonic hindlimb morphogenesis; intracellular transport; positive regulation of cAMP biosynthetic process; tissue homeostasis; DNA methylation; transmembrane transport; endochondral ossification; post-embryonic body morphogenesis; dopamine receptor, adenylate cyclase activating pathway; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; sensory perception of smell; adenylate cyclase activation; embryonic cranial skeleton morphogenesis; positive regulation of osteoblast differentiation; cartilage development; energy reserve metabolic process; renal water homeostasis; cAMP biosynthetic process; cognition; blood coagulation; regulation of insulin secretion

Disease: Osseous Heteroplasia, Progressive; Pseudopseudohypoparathyroidism; Pituitary Adenoma, Acth-secreting; Acth-independent Macronodular Adrenal Hyperplasia; Mccune-albright Syndrome; Pseudohypoparathyroidism, Type Ia; Pseudohypoparathyroidism, Type Ib; Pituitary Adenoma, Growth Hormone-secreting

Research Articles on GNAS

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Product Notes

The GNAS gnas (Catalog #AAA6302940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GNAS, CT (GNAS, GNAS1, Neuroendocrine secretory protein 55, LHAL tetrapeptide, GPIPIRRH peptide) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GNAS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GNAS gnas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNAS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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