Rabbit ACC1 Polyclonal Antibody | anti-ACC1 antibody

ACC1 (Phospho-Ser80) Antibody

Cat. Num. AAA9502404

• Gene Names: ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: ACC1; Polyclonal Antibody; ACC1 (Phospho-Ser80) Antibody; ACAC; ACACA; ACC-alpha; ACCA; Acetyl-CoA carboxylase 1; anti-ACC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: ACC1 (Phospho-Ser80) Antibody detects endogenous levels of ACC1 only when phosphorylated at Ser80.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2,383

• Applicable Applications for anti-ACC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:1000
• Target Modification: Phospho
• Modification Sites: H:S80; M:S79; R:S79
• Immunogen: The antiserum was produced against synthesized peptide derived from human ACC1 around the phosphorylation site of Ser80.
• Immunogen Range: 46-95
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACC1 (Phospho-Ser80) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from K562 cells treated with Insulin 0.01U/ml 15', using ACC1 (Phospho-Ser80) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 38679960
• NCBI GeneID: 31
• NCBI Accession #: NP_942131.1
• NCBI GenBank Nucleotide #: NM_198834.2
• UniProt Accession #: Q13085; Q6KEV6; Q6XDA8; Q7Z2G8; Q7Z561; Q7Z563; Q7Z564; Q86WB2; Q86WB3; B2RP68
• Molecular Weight: 265 kDa
• NCBI Official Full Name: acetyl-CoA carboxylase 1 isoform 1
• NCBI Official Synonym Full Names: acetyl-CoA carboxylase alpha
• NCBI Official Symbol: ACACA
• NCBI Official Synonym Symbols: ACC; ACAC; ACC1; ACCA; ACACAD
• NCBI Protein Information: acetyl-CoA carboxylase 1
• UniProt Protein Name: Acetyl-CoA carboxylase 1
• Protein Family: Acetyl-CoA carboxylase
• UniProt Gene Name: ACACA
• UniProt Synonym Gene Names: ACAC; ACC1; ACCA; ACC1

NCBI Description

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.