Mononucleosomes H3T3ph Recombinant Protein | H3T3ph recombinant protein

Recombinant Mononucleosomes H3T3ph (EPL)-biotin

Cat. Num. AAA389217

• Gene Names: HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
• Synonyms: Mononucleosomes H3T3ph; Recombinant Mononucleosomes H3T3ph (EPL)-biotin; H3T3ph recombinant protein

• Host: E Coli
• Form/Format: Recombinant Mononucleosomes H3T3pH(EPL)-biotin (20ug protein + 20ug DNA) are supplied at a protein concentration of 0.51mg/ml in 10mM Tris-HCl pH8.0, 1mM EDTA, 2mM DTT, and 20% glycerol.

• Protein Species: Human
• Conjugation: Biotinylated, Phosphorylated
• Notes: Recombinant Mononucleosomes H3T3ph (EPL)-biotin are suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant Mononucleosomes H3T3ph (EPL) - biotin SDS PAGE gel 13% SDS-PAGE, stained with Coomassie blue Purity: ? 92%)

Testing Data

(Recombinant Mononucleosomes H3T3ph (EPL) – biotin, DNA gel Mononucleosomes H3T3ph (EPL) - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact mononucleosome H3T3ph - biotin. Lane 3: 601 DNA. Intact mononucleosome H3T3ph - biotin migrated higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)

Western Blot (WB)

(Western Blot analysis for Recombinant Mononucleosomes H3T3ph (EPL) – biotin Recombinant Mononucleosomes H3T3ph (EPL) - biotin (Lane 1) and unmodified nucleosomes (Lane 2) were detected with H3T3ph antibody and H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3T3ph (EPL) - biotin can be detected by H3T3ph antibody.)

Related Product Information for H3T3ph recombinant protein

Short Description: Recombinant Mononucleosomes H3T3ph (EPL)-biotin consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NP_003503.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end)(accession number NP_066403.2) with phosphorylation at threonine 3, and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli. The molecular weight of histone octamer is ~108 kDa.

Background: Western Blot analysis for Recombinant Mononucleosomes H3T3ph (EPL)-biotin Recombinant Mononucleosomes H3T3ph (EPL)-biotin (Lane 1) and unmodified nucleosomes (Lane 2) were detected with H3T3ph antibody and H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3T3ph (EPL)-biotin can be detected by H3T3ph antibody.

Product Categories/Family for H3T3ph recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4504245
• NCBI GeneID: 8334
• NCBI Accession #: NP_003503.1; NP_003509.1; NP_066403.2; NP_003539.1
• NCBI GenBank Nucleotide #: NM_003512.3
• UniProt Accession #: Q93077; O00775; O00776; O00777; O00778; Q540R1; B2R4F7
• Molecular Weight: 14,105 Da
• NCBI Official Full Name: histone H2A type 1-C
• NCBI Official Synonym Full Names: histone cluster 1 H2A family member c
• NCBI Official Symbol: HIST1H2AC
• NCBI Official Synonym Symbols: H2A/l; H2AFL; dJ221C16.4
• NCBI Protein Information: histone H2A type 1-C
• UniProt Protein Name: Histone H2A type 1-C
• UniProt Gene Name: HIST1H2AC
• UniProt Synonym Gene Names: H2AFL

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.