Goat GNAQ Polyclonal Antibody | anti-GNAQ antibody

Goat Polyclonal to Human GNAQ

Cat. Num. AAA243948

• Gene Names: GNAQ; GAQ; SWS; CMC1; G-ALPHA-q
• Reactivity: Bat, Gibbon, Bovine, Dog, Human, Monkey, Pig, Rabbit, Rat; Predicted Reactivity: Mouse, Hamster, Horse (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GNAQ; Polyclonal Antibody; Goat Polyclonal to Human GNAQ; Anti-GNAQ Antibody (Internal) IHC-plus; G-ALPHA-q; GAQ; Human GNAQ; anti-GNAQ antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Human, Monkey, Pig, Rabbit, Rat; Predicted Reactivity: Mouse, Hamster, Horse (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GNAQ.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 359

• Applicable Applications for anti-GNAQ antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (5 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38 to 40kD band observed in Human, Mouse and Rat Testis lysates (calculated MW of 42.1kD according to Human NP_002063.2 and to Rat NP_112298.1). Recommended concentration...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-NDLDRVADPAYLPT from an internal region of human GNAQ (NP_002063.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Bat, Dog, Rabbit, Pig, Opossum (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: GNAQ antibody was raised against synthetic peptide C-NDLDRVADPAYLPT from an internal region of human GNAQ (NP_002063.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Bat, Dog, Rabbit, Pig, Opossum (100%); Mouse, Rat, Hamster, Horse, Platypus (93%); Drosophila (86%).
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-GNAQ antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(GNAQ antibody (0.1 ug/ml) staining of Rat Testis lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-GNAQ antibody

Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors.

NCBI and Uniprot Product Information

• NCBI GI #: 40254462
• NCBI GeneID: 2776
• NCBI Accession #: NP_002063.2
• NCBI GenBank Nucleotide #: NM_002072.4
• UniProt Accession #: P50148; O15108; Q13462; Q6NT27; Q92471; Q9BZB9
• Molecular Weight: 42,142 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(q) subunit alpha
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), q polypeptide
• NCBI Official Symbol: GNAQ
• NCBI Official Synonym Symbols: GAQ; SWS; CMC1; G-ALPHA-q
• NCBI Protein Information: guanine nucleotide-binding protein G(q) subunit alpha; guanine nucleotide-binding protein G(q) subunit alpha; guanine nucleotide-binding protein alpha-q
• UniProt Protein Name: Guanine nucleotide-binding protein G(q) subunit alpha
• UniProt Gene Name: GNAQ
• UniProt Synonym Gene Names: GAQ
• UniProt Entry Name: GNAQ_HUMAN

NCBI Description

This locus encodes a guanine nucleotide-binding protein. The encoded protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospolipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation. A related pseudogene exists on chromosome 2.[provided by RefSeq, Nov 2010]

Uniprot Description

G-alpha(q): a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein type: G protein, heterotrimeric alpha G(q); G protein, heterotrimeric; G protein

Chromosomal Location of Human Ortholog: 9q21

Cellular Component: nuclear membrane; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex

Molecular Function: GTPase activity; signal transducer activity; protein binding; GTP binding; type 2A serotonin receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; GTPase activator activity

Biological Process: platelet activation; regulation of action potential; protein stabilization; maternal behavior; metabolic process; glutamate signaling pathway; heart development; pigmentation during development; G-protein signaling, adenylate cyclase activating pathway; regulation of melanocyte differentiation; post-embryonic development; forebrain neuron development; phospholipase C activation; negative regulation of protein kinase activity; dopamine receptor, phospholipase C activating pathway; embryonic digit morphogenesis; blood coagulation; skeletal development; positive regulation of GTPase activity; neuron remodeling

Disease: Sturge-weber Syndrome; Capillary Malformations, Congenital