Western Blot (WB)
(Western blot analysis of lysates from Hela, HepG2 cell line, mouse liver and rat liver tissue lysate (from left to right), using POLD2 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. )
Rabbit POLD2 Polyclonal Antibody | anti-POLD2 antibody
POLD2 Antibody (Center)
Reactivity
Human, Mouse, RatPredicted: Bovine
Applications
Western Blot, ELISA
Synonyms
POLD2; Polyclonal Antibody; POLD2 Antibody (Center); DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; anti-POLD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Bovine
Predicted: Bovine
Clonality
Polyclonal
Isotype
Ig
Specificity
This POLD2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 232-265 amino acids from the Central region of human POLD2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
469
Applicable Applications for anti-POLD2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000
Antigen Source
Human
Antigen Region
232-265
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from Hela, HepG2 cell line, mouse liver and rat liver tissue lysate (from left to right), using POLD2 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. )
Western Blot (WB)
(Western blot analysis of lysates from Hela, HepG2 cell line, mouse liver and rat liver tissue lysate (from left to right), using POLD2 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. )
Related Product Information for anti-POLD2 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: The function of the small subunit is not yet clear.
Background: The function of the small subunit is not yet clear.
References
Zhang J.,et al.Genomics 29:179-186(1995). Perez A.,et al.Biochim. Biophys. Acta 1493:231-236(2000). He H.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:11979-11984(2001). Liu L.,et al.J. Biol. Chem. 278:10041-10047(2003). Tsurimoto T.,et al.Genes Cells 10:13-22(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51289
NCBI Official Full Name
DNA polymerase delta subunit 2 isoform 1
NCBI Official Synonym Full Names
DNA polymerase delta 2, accessory subunit
NCBI Official Symbol
POLD2
NCBI Protein Information
DNA polymerase delta subunit 2
UniProt Protein Name
DNA polymerase delta subunit 2
Protein Family
UniProt Gene Name
POLD2
UniProt Entry Name
DPOD2_HUMAN
NCBI Description
This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Mar 2012]
Research Articles on POLD2
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Product Notes
The POLD2 pold2 (Catalog #AAA9231708) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLD2 Antibody (Center) reacts with Human, Mouse, Rat Predicted: Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's POLD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000. Researchers should empirically determine the suitability of the POLD2 pold2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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