Typical Testing Data/Standard Curve (for reference only)
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Human Transcription factor MafB (MAFB) ELISA Kit | MAFB elisa kit
Human Transcription factor MafB (MAFB) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: MAFB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MAFB antibody and an MAFB-HRP conjugate. The assay sample and buffer are incubated together with MAFB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAFB concentration since MAFB from samples and MAFB-HRP conjugate compete for the anti-MAFB antibody binding site. Since the number of sites is limited, as more sites are occupied by MAFB from the sample, fewer sites are left to bind MAFB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAFB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns. [provided by RefSeq, Jul 2008]
Uniprot Description
MAFB: Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Defects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO). MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients. Belongs to the bZIP family. Maf subfamily.
Protein type: Oncoprotein; DNA-binding; Transcription factor; Tumor suppressor
Chromosomal Location of Human Ortholog: 20q12
Cellular Component: transcription factor complex; nucleus
Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: rhombomere 6 development; segment specification; negative regulation of erythrocyte differentiation; inner ear morphogenesis; sensory organ development; transcription, DNA-dependent; thymus development; T cell differentiation in the thymus; positive regulation of transcription from RNA polymerase II promoter; respiratory gaseous exchange; rhombomere 5 development; brain segmentation
Disease: Multicentric Carpotarsal Osteolysis Syndrome
Research Articles on MAFB
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Product Notes
The Human MAFB mafb (Catalog #AAA7207995) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7207995 ELISA Kit recognizes Human MAFB. It is sometimes possible for the material contained within the vial of "Transcription factor MafB (MAFB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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