Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 3ug/ml Rabbit Anti-Rat PAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Phosphoprotein Associated With Glycosphingolipid Microdomains 1 Polyclonal Antibody | anti-PAG1 antibody
Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1)
Gene Names
Pag1; Cbp
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Phosphoprotein Associated With Glycosphingolipid Microdomains 1; Polyclonal Antibody; Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1); anti-PAG1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PAG1. It has beenselected for its ability to recognize PAG1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IPP ENAVDAMLTA RAADGDSGPG VEGPYEVLKD SSSQENMVED CLYETVKEIK EVADKSQGGK SKSTSALKEL QGAHAEGKAD FAEYASVDRN KKCRHSTNAE SILGTSSDLD EETPPPVPVK LLDENANLPE KGEHGAEEQA PEAPSGHSKR FSSLSYKSRE EDPTLTEEEI SAMYSSVNKP GQSAHKPGPE
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IPP ENAVDAMLTA RAADGDSGPG VEGPYEVLKD SSSQENMVED CLYETVKEIK EVADKSQGGK SKSTSALKEL QGAHAEGKAD FAEYASVDRN KKCRHSTNAE SILGTSSDLD EETPPPVPVK LLDENANLPE KGEHGAEEQA PEAPSGHSKR FSSLSYKSRE EDPTLTEEEI SAMYSSVNKP GQSAHKPGPE
Applicable Applications for anti-PAG1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
PAG1 (Ile138~Phe357)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant PAG1 (Ile138~Phe357) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2078349
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 3ug/ml Rabbit Anti-Rat PAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 3ug/ml Rabbit Anti-Rat PAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded testis tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded testis tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,915 Da
NCBI Official Full Name
phosphoprotein associated with glycosphingolipid-enriched microdomains 1
NCBI Official Synonym Full Names
phosphoprotein membrane anchor with glycosphingolipid microdomains 1
NCBI Official Symbol
Pag1
NCBI Official Synonym Symbols
Cbp
NCBI Protein Information
phosphoprotein associated with glycosphingolipid-enriched microdomains 1
UniProt Protein Name
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Protein Family
UniProt Gene Name
Pag1
UniProt Synonym Gene Names
Cbp; Pag
NCBI Description
plays a role in negative regulation of FcepsilonRI signaling [RGD, Feb 2006]
Uniprot Description
PAG: an ubiquitous type III transmembrane adaptor protein that binds to the tyrosine kinase CSK protein. It is thought to be involved in the regulation of T cell activation.
Protein type: Adaptor/scaffold; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q23
Cellular Component: integral component of plasma membrane; intracellular; membrane raft; plasma membrane
Molecular Function: SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity
Biological Process: adaptive immune response; negative regulation of T cell activation; regulation of T cell activation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on PAG1
Similar Products
Product Notes
The PAG1 pag1 (Catalog #AAA2027903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoprotein Associated With Glycosphingolipid Microdomains 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the PAG1 pag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IPP ENAVDAMLTA RAADGDSGPG VEGPYEVLKD SSSQENMVED CLYETVKEIK EVADKSQGGK SKSTSALKEL QGAHAEGKAD FAEYASVDRN KKCRHSTNAE SILGTSSDLD EETPPPVPVK LLDENANLPE KGEHGAEEQA PEAPSGHSKR FSSLSYKSRE EDPTLTEEEI SAMYSSVNKP GQSAHKPGPE. It is sometimes possible for the material contained within the vial of "Phosphoprotein Associated With Glycosphingolipid Microdomains 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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