Rabbit anti-Rat Glutathione S Transferase Pi (GSTp) Polyclonal Antibody | anti-GSTp antibody

Polyclonal Antibody to Glutathione S Transferase Pi (GSTp)

Cat. Num. AAA2003702

• Gene Names: Gstp1; Gst3; Gstp; GST-P; Gstp2
• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Glutathione S Transferase Pi (GSTp); Polyclonal Antibody; Polyclonal Antibody to Glutathione S Transferase Pi (GSTp); anti-GSTp antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against GSTp. It has been selected for its ability to recognize GSTp in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGS-MPPYTIVYFP VRGRCEATRM LLADQGQSWK EEVVTIDVWL QGSLKSTCLY GQLPKFEDGD LTLYQSNAIL RHLGRSLGLY GKDQKEAALV DMVNDGVEDL RCKYGTLIYT NYENGKDDYV KALPGHLKPF ETLLSQNQGG KAFIVGNQIS FADYNLLDLL LVHQVLAPGC LDNFPLLSAY VARLSARPKI KAFLSSPDHL NRPINGNGKQ
• Sequence Length: 210

• Applicable Applications for anti-GSTp antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant GSTp (Met1~Gln210) expressed in E.coli.
• Cross Reactivity: Mouse, Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2051508
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Liver lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat GSTp Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Rat Serum; Primary Ab: 3ug/ml Rabbit Anti-Rat GSTp Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat GSTp Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat GSTp Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat GSTp Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)))

Western Blot (WB)

(Western Blot: Sample: Recombinant protein)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Testis Tissue))

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded liver tissue)

NCBI and Uniprot Product Information

• NCBI GI #: 25453420
• NCBI GeneID: 24426
• NCBI Accession #: NP_036709.1
• NCBI GenBank Nucleotide #: NM_012577.2
• UniProt Accession #: P04906
• Molecular Weight: 23,439 Da
• NCBI Official Full Name: glutathione S-transferase P
• NCBI Official Synonym Full Names: glutathione S-transferase pi 1
• NCBI Official Symbol: Gstp1
• NCBI Official Synonym Symbols: Gst3; Gstp; GST-P; Gstp2
• NCBI Protein Information: glutathione S-transferase P
• UniProt Protein Name: Glutathione S-transferase P
• UniProt Gene Name: Gstp1

NCBI Description

may play a role in drug and xenobiotic metabolism [RGD, Feb 2006]

Uniprot Description

GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family.

Protein type: EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytoplasm; cytosol; extracellular space; intracellular; mitochondrion; nucleus; plasma membrane; protein complex; vesicle

Molecular Function: drug binding; glutathione binding; glutathione peroxidase activity; glutathione transferase activity; JUN kinase binding; kinase regulator activity; protein kinase binding

Biological Process: cellular response to insulin stimulus; glutathione metabolic process; linoleic acid metabolic process; negative regulation of biosynthetic process; negative regulation of fibroblast proliferation; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of interleukin-1 beta production; negative regulation of JNK activity; negative regulation of MAP kinase activity; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of protein kinase activity; negative regulation of stress-activated MAPK cascade; negative regulation of tumor necrosis factor production; oligodendrocyte development; organ regeneration; positive regulation of superoxide anion generation; regulation of stress-activated MAPK cascade; response to amino acid; response to estradiol; response to ethanol; response to L-ascorbic acid; response to nutrient levels; response to reactive oxygen species; response to toxin; xenobiotic metabolic process