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Western Blot (WB) (Western Blot: Sample: Bovine Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Bovine GSTp Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Cattle Glutathione S Transferase Pi (GSTp) Polyclonal Antibody | anti-GSTp antibody

Polyclonal Antibody to Glutathione S Transferase Pi (GSTp)

Reactivity
Cattle
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Glutathione S Transferase Pi (GSTp); Polyclonal Antibody; Polyclonal Antibody to Glutathione S Transferase Pi (GSTp); anti-GSTp antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Cattle
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GSTp. It has been selected for its ability to recognize GSTp in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGS-MPPYTIVYFP VQGRCEAMRM LLADQGQSWK EEVVAMQSWL QGPLKASCLY GQLPKFQDGD LTLYQSNAIL RHLGRTLGLY GKDQQEAALV DMVNDGVEDL RCKYVSLIYT NYEAGKEDYV KALPQHLKPF ETLLSQNKGG QAFIVGDQIS FADYNLLDLL RIHQVLAPSC LDSFPLLSAY VARLNSRPKL KAFLASPEHM NRPINGNGKQ
Sequence Length
210
Applicable Applications for anti-GSTp antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GSTp (Met1~Gln210) expressed in E.coli.
Cross Reactivity
Bovine
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051481
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Bovine Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Bovine GSTp Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Bovine Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Bovine GSTp Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,613 Da
NCBI Official Full Name
glutathione S-transferase P
NCBI Official Symbol
GSTP1
NCBI Protein Information
glutathione S-transferase P
UniProt Protein Name
Glutathione S-transferase P
UniProt Gene Name
GSTP1

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration ().

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Product Notes

The GSTp gstp1 (Catalog #AAA2003526) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutathione S Transferase Pi (GSTp) reacts with Cattle and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione S Transferase Pi (GSTp) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GSTp gstp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG S-MPPYTIVY FP VQGRCEAMRM LLADQGQSWK EEVVAMQSWL QGPLKASCLY GQLPKFQDGD LTLYQSNAIL RHLGRTLGLY GKDQQEAALV DMVNDGVEDL RCKYVSLIYT NYEAGKEDYV KALPQHLKPF ETLLSQNKGG QAFIVGDQIS FADYNLLDLL RIHQVLAPSC LDSFPLLSAY VARLNSRPKL KAFLASPEHM NRPINGNGKQ. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase Pi (GSTp), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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