Rabbit anti-Human, Mouse Glutathione Peroxidase 3 Polyclonal Antibody | anti-GPX3 antibody

Glutathione Peroxidase 3, ID (GPX3, GSHPx-3, GPx-3, GSHPx-P, GPx-P, GPXP) (Biotin)

Cat. Num. AAA6304197

• Gene Names: GPX3; GPx-P; GSHPx-3; GSHPx-P
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Glutathione Peroxidase 3; Polyclonal Antibody; ID (GPX3; GSHPx-3; GPx-3; GSHPx-P; GPx-P; GPXP) (Biotin); GPX3; GPXP; Glutathione peroxidase 3; Extracellular glutathione peroxidase; Plasma glutathione peroxidase; anti-GPX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GPX3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-131 amino acids from the Central region of human GPX3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GPX3 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GPX3 antibody detected the GPX3 protein (arrow).)

Immunohistochemistry (IHC)

(GPX3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GPX3 antibody

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

Product Categories/Family for anti-GPX3 antibody

Antibodies; Enzymes; Peroxidase

References

Wang, J.Y., et al. Clin. Chim. Acta 411 (19-20), 1432-1436 (2010): Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010). Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010). Mistry, H.D., et al. Placenta 31(5):401-408(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 6006001
• NCBI GeneID: 2878
• NCBI Accession #: NP_002075
• NCBI GenBank Nucleotide #: NM_002084.4
• UniProt Accession #: P22352; O43787; Q86W78; Q9NZ74; Q9UEL1
• Molecular Weight: 25.7 kDa (227aa) confirmed by MALDI-TOF
• NCBI Official Full Name: glutathione peroxidase 3 isoform 1
• NCBI Official Synonym Full Names: glutathione peroxidase 3
• NCBI Official Symbol: GPX3
• NCBI Official Synonym Symbols: GPx-P; GSHPx-3; GSHPx-P
• NCBI Protein Information: glutathione peroxidase 3
• UniProt Protein Name: Glutathione peroxidase 3
• Protein Family: Glutathione peroxidase
• UniProt Gene Name: GPX3
• UniProt Synonym Gene Names: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
• UniProt Entry Name: GPX3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]

Uniprot Description

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Secreted; Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Oxidoreductase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding

Biological Process: response to reactive oxygen species; response to lipid hydroperoxide; hydrogen peroxide catabolic process; protein homotetramerization