Rabbit PTEN Polyclonal Antibody | anti-PTEN antibody

PTEN (S364) Polyclonal Antibody

Cat. Num. AAA3000826

• Gene Names: PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PTEN; Polyclonal Antibody; PTEN (S364) Polyclonal Antibody; Phosphatidylinositol 3; 4; 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; MMAC1; TEP1; anti-PTEN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PTEN (S364) polyclonal antibody detects endogenous levels of PTEN protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 403

• Applicable Applications for anti-PTEN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 350-400 of Human PTEN.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PTEN (S364) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Mouse spleen tissue lysateLane3:Rat spleen tissue lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PTEN (S364) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of PTEN (S364) pAb at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of PTEN (S364) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:The kidney tissue lysate of Mouse (40ug) Lane3:The kidney tissue lysate of Rat (40ug) Lane4:The lung tissue lysate of Mouse (40ug) Lane5:The lung tissue lysate of Rat (40ug))

Related Product Information for anti-PTEN antibody

As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10q23. Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN gene, also designated MMAC1 (for mutated in multiple advanced cancers) and TEP1. This candidate tumor suppressor gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers. PTEN has been associated with Cowden disease, an autosomal dominant cancer predisposition syndrome. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm, and it shares extensive homology with the cytoskeletal proteins tensin and auxilin. Gene transfer studies have indicated that the phosphatase domain of PTEN is essential for growth suppression of glioma cells.

NCBI and Uniprot Product Information

• NCBI GI #: 73765544
• NCBI GeneID: 5728
• NCBI Accession #: NP_000305.3
• NCBI GenBank Nucleotide #: NP_000305.3
• UniProt Accession #: P60484
• Molecular Weight: ~ 47, 54kDa
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN
• NCBI Official Synonym Full Names: phosphatase and tensin homolog
• NCBI Official Symbol: PTEN
• NCBI Official Synonym Symbols: BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase
• UniProt Gene Name: PTEN
• UniProt Synonym Gene Names: MMAC1; TEP1
• UniProt Entry Name: PTEN_HUMAN

NCBI Description

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]