Flow Cytometry (FC/FACS)
(GFP antibody is able to detect positive cell population in cytoflow analysis. A. cells were not stained with anti-GFP antibody/fluorescence complex. B. Cells were stained with GFP antibody/fluorescence complex and sorted.)
Mouse GFP Monoclonal Antibody | anti-GFP antibody
GFP antibody
IHC: 1:1,000-1:5,000
WB: 1:3,000-1:30,000
Flow Cytometry (FC/FACS)
(GFP antibody is able to detect positive cell population in cytoflow analysis. A. cells were not stained with anti-GFP antibody/fluorescence complex. B. Cells were stained with GFP antibody/fluorescence complex and sorted.)
Flow Cytometry (FC/FACS)
(GFP antibody is able to detect positive cell population in cytoflow analysis. A. cells were not stained with anti-GFP antibody/fluorescence complex. B. Cells were stained with GFP antibody/fluorescence complex and sorted.)
Western Blot (WB)
(Western Blot of GFP antibody. Lane 1: HeLa lysate 50 ng. Lane 2: HeLa lysate 100 ng. Lane 3: HeLa lysate 500 ng. GFP antibody at 1.0 mg/ml for 1 h at room temperature. IRDye800 conjugated Goat-a-Mouse IgG [H&L] at 1:2,500 dilution for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 27 kDa for epitope tag GFP..)
Western Blot (WB)
(Western Blot of GFP antibody. Lane 1: HeLa lysate 50 ng. Lane 2: HeLa lysate 100 ng. Lane 3: HeLa lysate 500 ng. GFP antibody at 1.0 mg/ml for 1 h at room temperature. IRDye800 conjugated Goat-a-Mouse IgG [H&L] at 1:2,500 dilution for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 27 kDa for epitope tag GFP..)
Western Blot (WB)
(Western Blot of GFP antibody. Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Ms Anti-GFP antibody at 1:1000 for overnight at 4 deg C. Mouse HRP secondary antibody at 1:40,000 for 30 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 27 kDa for epitope tag GFP..)
Western Blot (WB)
(Western Blot of GFP antibody. Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Ms Anti-GFP antibody at 1:1000 for overnight at 4 deg C. Mouse HRP secondary antibody at 1:40,000 for 30 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 27 kDa for epitope tag GFP..)
Western Blot (WB)
(Western Blot of GFP antibody. Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. anti-GFP monoclonal antibody at 1:400 for overnight at 4 deg C. HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4 deg C. Block: TTBS supplemented with 1% BSA for 1 h at 4 deg C. Size: 27 kDa for GFP..)
Western Blot (WB)
(Western Blot of GFP antibody. Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. anti-GFP monoclonal antibody at 1:400 for overnight at 4 deg C. HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4 deg C. Block: TTBS supplemented with 1% BSA for 1 h at 4 deg C. Size: 27 kDa for GFP..)
NCBI and Uniprot Product Information
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Product Notes
The GFP (Catalog #AAA531060) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GFP antibody reacts with Jellyfish GFP and may cross-react with other species as described in the data sheet. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:200,000 IHC: 1:1,000-1:5,000 WB: 1:3,000-1:30,000. Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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