Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: MOLT-4 whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit GLUL Polyclonal Antibody | anti-GLUL antibody
GLUL Antibody (N-term)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: MOLT-4 whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: MOLT-4 whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: Hela whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: Hela whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: Hela whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: Hela whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-GLUL Antibody (N-term)at 1:1000 dilution + Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-GLUL Antibody (N-term)at 1:1000 dilution + Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: mouse cerebellum lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: mouse cerebellum lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: HT-29 whole cell lysatesLane 2: mouse cerebellum lysatesLane 3: Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: HT-29 whole cell lysatesLane 2: mouse cerebellum lysatesLane 3: Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse cerebellum lysatesLane 2: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilutionLane 1: mouse cerebellum lysatesLane 2: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Uniprot Description
GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family.
Protein type: Ligase; EC 4.1.1.15; EC 6.3.1.2; Amino Acid Metabolism - alanine, aspartate and glutamate; Energy Metabolism - nitrogen; Amino Acid Metabolism - arginine and proline
Chromosomal Location of Human Ortholog: 1q31
Cellular Component: protein complex; rough endoplasmic reticulum; mitochondrion; cytoplasm; perikaryon; nerve terminal; nucleus; cytosol
Molecular Function: glutamate-ammonia ligase activity; identical protein binding; dynein light chain binding; glutamate binding; glutamate decarboxylase activity; manganese ion binding; magnesium ion binding; ATP binding
Biological Process: cell proliferation; glutamate catabolic process; synaptic transmission; glutamine biosynthetic process; response to glucose stimulus; positive regulation of insulin secretion; neurotransmitter uptake; amino acid biosynthetic process; cellular response to starvation; positive regulation of synaptic transmission, glutamatergic; positive regulation of epithelial cell proliferation; protein homooligomerization
Disease: Glutamine Deficiency, Congenital
Research Articles on GLUL
Similar Products
Product Notes
The GLUL glul (Catalog #AAA9204046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 70-96. The GLUL Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Chicken, Monkey, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's GLUL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the GLUL glul for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLUL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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