Rabbit Glutamate-Oxaloacetate Transaminase Polyclonal Antibody | anti-GOT1 antibody

Glutamate-Oxaloacetate Transaminase (GOT)

Cat. Num. AAA648293

• Gene Names: GOT1; cCAT; GIG18; cAspAT; ASTQTL1
• Applications: ELISA, Western Blot
• Purity: Highly Purified; Purified by ammonium sulphate precipitation and ion exchange chromatography.
• Synonyms: Glutamate-Oxaloacetate Transaminase; Polyclonal Antibody; Glutamate-Oxaloacetate Transaminase (GOT); Anti -Glutamate-Oxaloacetate Transaminase (GOT); anti-GOT1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Glutamate-Oxaloacetate Transaminase.
• Purity/Purification: Highly Purified; Purified by ammonium sulphate precipitation and ion exchange chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 1ml sterile ddH2O.

• Applicable Applications for anti-GOT1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Immunoelectrophoresis, RID, ELISA, Western Blot and Inhibition.
• Immunogen: Glutamate-oxaloacetate transaminase isolated and purified from porcine heart.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-GOT1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 3452380
• NCBI GeneID: 2805
• UniProt Accession #: P17174; Q5VW80; B2R6R7
• Molecular Weight: 46,248 Da
• NCBI Official Full Name: glutamate oxaloacetate transaminase
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1, soluble
• NCBI Official Symbol: GOT1
• NCBI Official Synonym Symbols: cCAT; GIG18; cAspAT; ASTQTL1
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic; transaminase A; aspartate transaminase 1; aspartate aminotransferase 1; growth-inhibiting protein 18; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; cysteine aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Protein transport protein
• UniProt Gene Name: GOT1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. Ref.9

Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.L-cysteine + 2-oxoglutarate = mercaptopyruvate + L-glutamate.

Cofactor: Pyridoxal phosphate.

Subunit structure: Homodimer. Ref.13

Subcellular location: Cytoplasm Ref.1.

Polymorphism: Genetic variations in GOT1 are associated with low serum aspartate aminotransferase and define the aspartate aminotransferase serum level quantitative trait locus 1 (ASTQTL1) [

MIM:614419].

Miscellaneous: In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.Aspartate aminotransferase activity found to be increased in cerebral spinal fluid (CSF) of patients with Alzheimer disease (Ref.9). Fetal serum levels of the enzyme in the umbilical artery and vein are found to be significantly higher than maternal serum levels (Ref.12).

Sequence similarities: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.