Rabbit GluR2 Polyclonal Antibody | anti-GluR2 antibody

Phospho-GluR2 (Tyr876) Antibody

Cat. Num. AAA9614473

• Gene Names: GRIA2; GLUR2; GLURB; GluA2; HBGR2; GluR-K2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: GluR2; Polyclonal Antibody; Phospho-GluR2 (Tyr876) Antibody; AMPA 2; AMPA selective glutamate receptor 2; AMPA-selective glutamate receptor 2; AMPA2; GluA2; GLUR 2; GLUR B; GluR K2; GluR-2; GluR-B; GluR-K2; GLUR2; GLURB; Glutamate receptor 2; Glutamate receptor ionotropic AMPA 2; Glutamate receptor ionotropic; Gria2; GRIA2_HUMAN; HBGR2; anti-GluR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-GluR2 (Tyr876) Antibody detects endogenous levels of GluR2 only when phosphorylated at Tyr876.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GluR2 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human GluR2 around the phosphorylation site of Tyr876.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
• Subunit Structure: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4. Interacts with ATAD1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts binding to ATAD1 and to GRIP1 and leads to AMPAR complex disassembly. Interacts with GRIP2. Isoform 1, but not isoform 3, interacts with PICK1/PRKCABP. Interacts with GRIA1 and SYNDIG1. Interacts with LRFN1 (By similarity). Found in a complex with GRIA1, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5 (By similarity). Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts with OLFM2 (By similarity). Interacts with AP4B1, AP4E1 and AP4M1; probably indirect it mediates the somatodendritic localization of GRIA2 in neurons (By similarity). Forms a complex with NSG1, GRIP1 and STX12; controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).
• Similarity: The M4 transmembrane segment mediates tetramerization and is required for cell surface expression.Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
• Subcellular Location: Cell membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein.; Note: Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression (By similarity). Displays a somatodendritic localization and is excluded from axons in neurons (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis GluR2 (Phospho-Tyr876) using K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-GluR2 antibody

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 74275354
• NCBI GeneID: 2891
• NCBI Accession #: NP_000817.2
• NCBI GenBank Nucleotide #: NM_000826.3
• UniProt Accession #: P42262; Q96FP6
• Molecular Weight: 98,821 Da
• NCBI Official Full Name: glutamate receptor 2 isoform 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA 2
• NCBI Official Symbol: GRIA2
• NCBI Official Synonym Symbols: GLUR2; GLURB; GluA2; HBGR2; GluR-K2
• NCBI Protein Information: glutamate receptor 2; gluR-2; gluR-B; AMPA-selective glutamate receptor 2
• UniProt Protein Name: Glutamate receptor 2
• UniProt Gene Name: GRIA2
• UniProt Synonym Gene Names: GLUR2; GluR-2; GluA2
• UniProt Entry Name: GRIA2_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR2: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA(quisqualate) > glutamate > kainate. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Channel, calcium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q32.1

Cellular Component: postsynaptic membrane; synaptic vesicle; endoplasmic reticulum membrane; integral to plasma membrane; dendrite; plasma membrane; cell junction

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Biological Process: synaptic transmission; synaptic transmission, glutamatergic; ionotropic glutamate receptor signaling pathway; signal transduction