Rabbit AMP Activated Protein Kinase alpha1 Polyclonal Antibody | anti-AMPKa1 antibody

AMP Activated Protein Kinase alpha1 (AMPK a1) (HRP)

Cat. Num. AAA6462682

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AMP Activated Protein Kinase alpha1; Polyclonal Antibody; AMP Activated Protein Kinase alpha1 (AMPK a1) (HRP); anti-AMPKa1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AMPK a1, Mr 63kD. Species Crossreactivity: rat and mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-AMPKa1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.; WB: 0.5-2ug/ml detected AMP-activated Protein Kinase a1 in RIPA lysates from human non-stimulated A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with.
• Immunogen: Synthetic peptide corresponding to aa376-392, C-ARHTLDELNPQKSKHQG-COOH of human AMP-activated Protein Kinase alpha1, conjugated to KLH (NP_006242).
• Conjugate: HRP
• Included Positive Antigen Control: MBS652617: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(WB: Non-stimulated A431 cell lysate was probed withMBS610694. Lane 1: Antibody alone. Lane 2: Antibody incubated with immunogenic peptide.)

Product Categories/Family for anti-AMPKa1 antibody

Antibodies; Protein Kinases

References

1. Phoenix, K.N. et al., Breast Cancer Res Treat. (2009). 113;101-111. 2. Neurath, K.M. et al., (2006). Glia 53:733-743. 3. Phoenix, K. N. et al., (2012) Genes & Cancer, doi: 10.1177/1947601912452883. 1. Chen, Z-P., et al., FEBS Letts. 443: 285-289 (1999). 2. Chen, Z-P., et al., FEBS Letts. 460: 343-348 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 94557301
• NCBI GeneID: 5562
• NCBI Accession #: NP_006242
• NCBI GenBank Nucleotide #: NM_006251.5
• UniProt Accession #: Q13131; O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1
• Molecular Weight: 34.3kDa (302aa)
• NCBI Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
• NCBI Official Synonym Full Names: protein kinase AMP-activated catalytic subunit alpha 1
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]