Mouse anti-Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody | anti-ATP1a1 antibody

Monoclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)

Cat. Num. AAA2111701

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Protein A + Protein G Affinity Chromatography
• Synonyms: ATPase; Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1); Monoclonal Antibody; Monoclonal Antibody to ATPase; Sodium pump subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium/potassium-transporting ATPase subunit alpha-1; anti-ATP1a1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: The antibody is a mouse monoclonal antibody raised against ATP1a1. It has been selected for its ability to recognize ATP1a1 in immunohistochemical staining and western blotting.
• Purity/Purification: Protein A + Protein G Affinity Chromatography
• Form/Format: Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence: His493~Ala660

• Applicable Applications for anti-ATP1a1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 0.2-2ug/mL; 1:500-5000; IHC: 5-20ug/mL; 1:50-200
• Immunogen Sequence: His493~Ala660
• Cross Reactivity: Human
• Conjugation: None
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Mouse Heart lysate; Primary Ab: 5ug/ml Mouse Anti-Human ATP1a1 Antibody Secon)

Western Blot (WB)

(Western Blot; Sample: Human A549 cell lysate; Primary Ab: 5ug/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Porcine Heart lysate; Lane3: Rat Heart lysate; Lane4: Bovine Heart lysate; Lane5: Rabbit Heart lysate; Lane6: Porcine Cerebrum lysatePrimary Ab: 2?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse Anti-Human ATP1a1 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human amygdalitis Tissue; Primary Ab: 10?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Ovarian cancer Tissue; Primary Ab: 10?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Product Categories/Family for anti-ATP1a1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NM_000701.7
• UniProt Accession #: P05023; Q16689; Q6LDM4; Q9UCN1; Q9UJ20; Q9UJ21; B2RBR6; B7Z3U6
• Molecular Weight: 112,896 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• NCBI Official Symbol: ATP1A1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1; sodium pump 1; Na+/K+ ATPase 1; Na,K-ATPase alpha-1 subunit; sodium pump subunit alpha-1; Na+, K+ ATPase alpha subunit; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; s
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit
• UniProt Entry Name: AT1A1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Transporter, ion channel; EC 3.6.3.9; Membrane protein, multi-pass; Hydrolase; Transporter

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: Golgi apparatus; protein complex; basolateral plasma membrane; endoplasmic reticulum; T-tubule; integral to membrane; caveola; membrane; apical plasma membrane; plasma membrane; melanosome; sodium:potassium-exchanging ATPase complex; sarcolemma; endosome; vesicle

Molecular Function: potassium ion binding; protein domain specific binding; protein binding; sodium ion binding; chaperone binding; phosphoric monoester hydrolase activity; sodium:potassium-exchanging ATPase activity; phosphoinositide 3-kinase binding; ankyrin binding; ADP binding; protein kinase binding; ATP binding

Biological Process: response to drug; cellular sodium ion homeostasis; negative regulation of heart contraction; membrane hyperpolarization; positive regulation of striated muscle contraction; regulation of sodium ion transport; cellular potassium ion homeostasis; negative regulation of glucocorticoid biosynthetic process; dephosphorylation; potassium ion import; regulation of blood pressure; positive regulation of heart contraction; transmembrane transport; regulation of the force of heart contraction; cardiac muscle contraction