Rabbit GluR1 Polyclonal Antibody | anti-GluR1 antibody

GluR1 Antibody

Cat. Num. AAA9613438

• Gene Names: GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: GluR1; Polyclonal Antibody; GluR1 Antibody; GLUR 1; GLUR A; AMPA 1; AMPA selective glutamate receptor 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR K1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; GluRK1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; Glutamate receptor ionotropic; Glutamate receptor; ionotropic; Gria1; GRIA1_HUMAN; HBGR1; MGC133252; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; anti-GluR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: GluR1 Antibody detects endogenous levels of total GluR1.; Tissue Specificity: Widely expressed in brain.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GluR1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human GluR1, corresponding to a region within C-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2 (By similarity). Phosphorylation of Ser-863 is reduced by induction of long-term depression and increased by induction of long-term potentiation (By similarity).
• Subunit Structure: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits (By similarity). Tetramers may be formed by the dimerization of dimers. Interacts with HIP1 and RASGRF2. Interacts with SYNDIG1 and GRIA2 (By similarity). Interacts with DLG1 (via C-terminus). Interacts with LRFN1. Interacts with PRKG2 (By similarity). Interacts with CNIH2 and CACNG2. Interacts with CACNG5. Interacts (via C-terminus) with PDLIM4 (via LIM domain); this interaction as well as the interaction of PDLIM4 with alpha-actinin is required for their colocalization in early endosomes. Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8 (By similarity). Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain) (By similarity). Interacts with CACNG3; associates GRIA1 with the adaptor protein complex 4 (AP-4) to target GRIA1 to the somatodendritic compartment of neurons (By similarity).
• Similarity: The M4 transmembrane segment mediates tetramerization and is required for cell surface expression.Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
• Subcellular Location: Cell membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic density membrane>Multi-pass membrane protein. Cell projection>Dendrite. Cell projection>Dendritic spine. Early endosome membrane>Multi-pass membrane protein. Recycling endosome membrane>Multi-pass membrane protein.; Note: Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Colocalizes with PDLIM4 in early endosomes. Displays a somatodendritic localization and is excluded from axons in neurons (By similarity). Localized to cone photoreceptor pedicles (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of AMPA Receptor in lysates of COLO205 , using AMPA Receptor Antibody(AF4730).)

Related Product Information for anti-GluR1 antibody

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

NCBI and Uniprot Product Information

• NCBI GI #: 167001419
• NCBI GeneID: 2890
• NCBI Accession #: NP_000818.2
• NCBI GenBank Nucleotide #: NM_000827.3
• UniProt Accession #: P42261; Q2NKM6; B7Z2S0; B7Z3F6; D3DQI4
• Molecular Weight: 101,506 Da
• NCBI Official Full Name: glutamate receptor 1 isoform 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA 1
• NCBI Official Symbol: GRIA1
• NCBI Official Synonym Symbols: GLUH1; GLUR1; GLURA; GluA1; HBGR1
• NCBI Protein Information: glutamate receptor 1; AMPA 1; gluR-1; gluR-A; gluR-K1; AMPA-selective glutamate receptor 1
• UniProt Protein Name: Glutamate receptor 1
• UniProt Gene Name: GRIA1
• UniProt Synonym Gene Names: GLUH1; GLUR1; GluR-1; GluA1
• UniProt Entry Name: GRIA1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Membrane protein, integral; Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: postsynaptic membrane; synaptic vesicle; endoplasmic reticulum membrane; recycling endosome; cell surface; cell soma; postsynaptic density; dendrite; dendritic spine; plasma membrane; cell junction

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; glutamate receptor activity; PDZ domain binding

Biological Process: synaptic transmission, glutamatergic; synaptic transmission; long-term memory; ionotropic glutamate receptor signaling pathway; receptor internalization; signal transduction