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Western Blot (WB) (Western blot analysis of AMPA Receptor in lysates of COLO205 , using AMPA Receptor Antibody(AF4730).)

Rabbit GluR1 Polyclonal Antibody | anti-GluR1 antibody

GluR1 Antibody

Gene Names
GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%)
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
GluR1; Polyclonal Antibody; GluR1 Antibody; GLUR 1; GLUR A; AMPA 1; AMPA selective glutamate receptor 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR K1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; GluRK1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; Glutamate receptor ionotropic; Glutamate receptor; ionotropic; Gria1; GRIA1_HUMAN; HBGR1; MGC133252; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; anti-GluR1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
GluR1 Antibody detects endogenous levels of total GluR1.
Tissue Specificity: Widely expressed in brain.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GluR1 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human GluR1, corresponding to a region within C-terminal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2 (By similarity). Phosphorylation of Ser-863 is reduced by induction of long-term depression and increased by induction of long-term potentiation (By similarity).
Subunit Structure
Homotetramer or heterotetramer of pore-forming glutamate receptor subunits (By similarity). Tetramers may be formed by the dimerization of dimers. Interacts with HIP1 and RASGRF2. Interacts with SYNDIG1 and GRIA2 (By similarity). Interacts with DLG1 (via C-terminus). Interacts with LRFN1. Interacts with PRKG2 (By similarity). Interacts with CNIH2 and CACNG2. Interacts with CACNG5. Interacts (via C-terminus) with PDLIM4 (via LIM domain); this interaction as well as the interaction of PDLIM4 with alpha-actinin is required for their colocalization in early endosomes. Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8 (By similarity). Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain) (By similarity). Interacts with CACNG3; associates GRIA1 with the adaptor protein complex 4 (AP-4) to target GRIA1 to the somatodendritic compartment of neurons (By similarity).
Similarity
The M4 transmembrane segment mediates tetramerization and is required for cell surface expression.Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
Subcellular Location
Cell membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic density membrane>Multi-pass membrane protein. Cell projection>Dendrite. Cell projection>Dendritic spine. Early endosome membrane>Multi-pass membrane protein. Recycling endosome membrane>Multi-pass membrane protein.
Note: Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Colocalizes with PDLIM4 in early endosomes. Displays a somatodendritic localization and is excluded from axons in neurons (By similarity). Localized to cone photoreceptor pedicles (By similarity).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of AMPA Receptor in lysates of COLO205 , using AMPA Receptor Antibody(AF4730).)

Western Blot (WB) (Western blot analysis of AMPA Receptor in lysates of COLO205 , using AMPA Receptor Antibody(AF4730).)
Related Product Information for anti-GluR1 antibody
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
101,506 Da
NCBI Official Full Name
glutamate receptor 1 isoform 1
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, AMPA 1
NCBI Official Symbol
GRIA1
NCBI Official Synonym Symbols
GLUH1; GLUR1; GLURA; GluA1; HBGR1
NCBI Protein Information
glutamate receptor 1; AMPA 1; gluR-1; gluR-A; gluR-K1; AMPA-selective glutamate receptor 1
UniProt Protein Name
Glutamate receptor 1
UniProt Gene Name
GRIA1
UniProt Synonym Gene Names
GLUH1; GLUR1; GluR-1; GluA1
UniProt Entry Name
GRIA1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Membrane protein, integral; Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: postsynaptic membrane; synaptic vesicle; endoplasmic reticulum membrane; recycling endosome; cell surface; cell soma; postsynaptic density; dendrite; dendritic spine; plasma membrane; cell junction

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; glutamate receptor activity; PDZ domain binding

Biological Process: synaptic transmission, glutamatergic; synaptic transmission; long-term memory; ionotropic glutamate receptor signaling pathway; receptor internalization; signal transduction

Research Articles on GluR1

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Product Notes

The GluR1 gria1 (Catalog #AAA9613438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GluR1 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's GluR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the GluR1 gria1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GluR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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