Rabbit GluR1 Polyclonal Antibody | anti-GluR1 antibody

RABBIT ANTI GluR1 (pSer831)

Cat. Num. AAA221804

• Gene Names: Gria1; GluA1; gluR-A
• Reactivity: Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence)
• Applications: Western Blot
• Synonyms: GluR1; Polyclonal Antibody; RABBIT ANTI GluR1 (pSer831); anti-GluR1 antibody

• Host: Rabbit
• Reactivity: Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for rat glutamate receptor subunit 1 (GluR1) when phosphorylated at serine 831. GluR1, widely expressed throughout the nervous system, is a component of AMPA receptors (alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid receptors), which play a key role at excitatory synapses. Phosphorylation of Ser831 and Ser845 is reported to potentiate GluR1 function, suggesting a possible role in synaptic plasticity, learning and memory.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 907

• Applicable Applications for anti-GluR1 antibody: Western Blot (WB)*
• Application Notes: Western Blot: This item detects a band of approximately 100 kDa in rat brain lysates.; Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Perservative Stabilisers: 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
• Immunogen: Synthetic phosphopeptide corresponding to amino acid sequence within GluR1 which includes phosphorylated Ser831.; Buffer Solution
• Antiserum Preparation: Antisera to phosphorylated GluR1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by sequential affinity chromatography on phospho- and dephosphopeptide affinity columns.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-GluR1 antibody

This antibody is specific for rat glutamate receptor subunit 1 (GluR1) when phosphorylated at serine 831. GluR1, widely expressed throughout the nervous system, is a component of AMPA receptors (alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid receptors), which play a key role at excitatory synapses. Phosphorylation of Ser831 and Ser845 is reported to potentiate GluR1 function, suggesting a possible role in synaptic plasticity, learning and memory.

NCBI and Uniprot Product Information

• NCBI GI #: 29789269
• NCBI GeneID: 50592
• NCBI Accession #: NP_113796.1
• NCBI GenBank Nucleotide #: NM_031608.1
• UniProt Accession #: P19490
• Molecular Weight: 101,555 Da
• NCBI Official Full Name: glutamate receptor 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA 1
• NCBI Official Symbol: Gria1
• NCBI Official Synonym Symbols: GluA1; gluR-A
• NCBI Protein Information: glutamate receptor 1; AMPA-selective glutamate receptor 1; GluR1; gluR-1; gluR-K1; glutamate receptor A; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA1 (alpha 1)
• UniProt Protein Name: Glutamate receptor 1
• UniProt Gene Name: Gria1
• UniProt Synonym Gene Names: Glur1; GluR-1; GluA1
• UniProt Entry Name: GRIA1_RAT

NCBI Description

mouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning [RGD, Feb 2006]

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated

Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cell surface; endoplasmic reticulum; early endosome; postsynaptic density; dendrite; integral to membrane; dendritic spine; excitatory synapse; cytosol; asymmetric synapse; postsynaptic membrane; recycling endosome; synaptic vesicle; membrane; cell soma; plasma membrane; ionotropic glutamate receptor complex; synapse; dendritic shaft; neuromuscular junction; cell junction

Molecular Function: identical protein binding; protein domain specific binding; protein homodimerization activity; myosin V binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; protein kinase binding; PDZ domain binding; small GTPase binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; beta-2 adrenergic receptor binding; protein kinase A binding; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; adenylate cyclase binding

Biological Process: regulation of synaptic transmission; response to drug; response to peptide hormone stimulus; synaptic transmission, glutamatergic; response to toxin; response to lithium ion; response to arsenic; generation of action potential; regulation of synaptic plasticity; response to cocaine; response to organic cyclic substance; positive regulation of synaptic transmission; response to estradiol stimulus; synaptic transmission; long-term memory; spinal cord development; ionotropic glutamate receptor signaling pathway; receptor internalization; regulation of receptor recycling; response to electrical stimulus; positive regulation of membrane potential