General Taurocholic Acid ELISA Kit | TCA elisa kit
General Taurocholic Acid (TCA) ELISA Kit
Reactivity
General
Synonyms
Taurocholic Acid; General Taurocholic Acid (TCA) ELISA Kit; Cholaic Acid; Cholyltaurine; Acidum Cholatauricum; TCA elisa kit
Reactivity
General
Specificity
This assay has high sensitivity and excellent specificity for detection of taurocholic acid. No significant cross-reactivity or interference between taurocholic acid and analogues was observed.
Samples
Biological fluids
Assay Type
Quantitative Competitive
Detection Range
3.70-300ng/mL
Sensitivity
< 1.33ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level taurocholic acid were tested 20 times on one plate, respectively.Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level taurocholic acid were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TCA elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of taurocholic acid in biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to taurocholic acid has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled taurocholic acid and unlabeled taurocholic acid (Standards or samples) with the pre-coated antibody specific to taurocholic acid. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of taurocholic acid in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of taurocholic acid in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to taurocholic acid has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled taurocholic acid and unlabeled taurocholic acid (Standards or samples) with the pre-coated antibody specific to taurocholic acid. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of taurocholic acid in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of taurocholic acid in the sample.
