Mouse anti-Rat, Mouse GluA1/GluR1 Glutamate Receptor Monoclonal Antibody | anti-Gria1 antibody

GluA1/GluR1 Glutamate Receptor Antibody: ATTO 488

Cat. Num. AAA800593

• Gene Names: Gria1; GluA1; gluR-A
• Reactivity: Rat, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Synonyms: GluA1/GluR1 Glutamate Receptor; Monoclonal Antibody; GluA1/GluR1 Glutamate Receptor Antibody: ATTO 488; AMPA 1; AMPA selective glutamate receptor 1; Glutamate receptor 1; GluA1; GLUH 1; GluR K1; GluRK1; Glr-1; Glur-1; HIPA; Glur1; GluR-A; AI853806; 2900051M01Rik; GluRA; Glr1; MGC13325; GLUH1; GLUR1; HBGR1; GLURA; gluR-; anti-Gria1 antibody

• Host: Mouse
• Reactivity: Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S355-1
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 907

• Applicable Applications for anti-Gria1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: 1:1000 WB
• Conjugate: ATTO 488
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Preparation and Storage: -20 degree C

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody, Clone S355-1 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluA1/GluR1 Glutamate Receptor Antibody (D) Composite.)

Western Blot (WB)

(Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluA1-GluR1 protein using Mouse Anti-GluA1-GluR1 Monoclonal Antibody, Clone S355-1 . Load: 10 ug. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluA1-GluR1 Monoclonal Antibody at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.)

Related Product Information for anti-Gria1 antibody

Background Info: Detects ~100kDa. Does not cross-react with GluR2.

Scientific Background: Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration (3).

Product Categories/Family for anti-Gria1 antibody

Cell Signaling; Neuroscience

References

1. Potier M.C., Spillantini M.G., Carter N.P. (1992) DNA Seq. 2(4): 211-218. 2. Puckett, C., et al. (1991) Proc. Nat. Acad. Sci. USA 88(17): 7557-7561. 3. Gregor, P., et al. (1993) Proc. Nat. Acad. Sci. USA 90: 3053-3057.

NCBI and Uniprot Product Information

• NCBI GI #: 29789269
• NCBI GeneID: 50592
• NCBI Accession #: NP_113796
• NCBI GenBank Nucleotide #: NM_031608.1
• UniProt Accession #: P19490
• Molecular Weight: 101,555 Da
• NCBI Official Full Name: glutamate receptor 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA 1
• NCBI Official Symbol: Gria1
• NCBI Official Synonym Symbols: GluA1; gluR-A
• NCBI Protein Information: glutamate receptor 1; GluR1; gluR-1; gluR-K1; glutamate receptor A; glutamate receptor subunit GluR1; AMPA-selective glutamate receptor 1; glutamate receptor, ionotropic, AMPA1 (alpha 1)
• UniProt Protein Name: Glutamate receptor 1
• UniProt Gene Name: Gria1
• UniProt Synonym Gene Names: Glur1; GluR-1; GluA1
• UniProt Entry Name: GRIA1_RAT

NCBI Description

mouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning [RGD, Feb 2006]

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Channel, calcium; Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cell surface; endoplasmic reticulum; postsynaptic density; dendrite; early endosome; integral to membrane; dendritic spine; excitatory synapse; cytosol; asymmetric synapse; postsynaptic membrane; synaptic vesicle; recycling endosome; membrane; cell soma; plasma membrane; ionotropic glutamate receptor complex; synapse; dendritic shaft; neuromuscular junction; cell junction

Molecular Function: identical protein binding; protein domain specific binding; protein homodimerization activity; myosin V binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; protein kinase binding; PDZ domain binding; small GTPase binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; beta-2 adrenergic receptor binding; protein kinase A binding; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; adenylate cyclase binding

Biological Process: response to drug; regulation of synaptic transmission; response to peptide hormone stimulus; synaptic transmission, glutamatergic; response to lithium ion; response to arsenic; response to toxin; generation of action potential; regulation of synaptic plasticity; response to cocaine; response to organic cyclic substance; positive regulation of synaptic transmission; response to estradiol stimulus; synaptic transmission; long-term memory; spinal cord development; ionotropic glutamate receptor signaling pathway; receptor internalization; regulation of receptor recycling; positive regulation of membrane potential; response to electrical stimulus