Western Blot (WB)
(Western Blot (WB) analysis of GLUD2 polyclonal antibody)
Rabbit GLUD2 Polyclonal Antibody | anti-GLUD2 antibody
GLUD2 Polyclonal Antibody
Gene Names
GLUD2; GDH2; GLUDP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
GLUD2; Polyclonal Antibody; GLUD2 Polyclonal Antibody; Glutamate dehydrogenase 2; mitochondrial; GDH 2; GLUDP1; anti-GLUD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GLUD2 polyclonal antibody detects endogenous levels of GLUD2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
558
Applicable Applications for anti-GLUD2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:10-1:100
IHC: 1:50-1:200
IF: 1:10-1:100
Immunogen
Recombinant full length Human GLUD2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of GLUD2 polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of GLUD2 polyclonal antibody)
Related Product Information for anti-GLUD2 antibody
GLUD1 (glutamate dehydrogenase 1), also known as GDH, GDH1 or GLUD, and GLUD2 (glutamate dehydrogenase 2), also known as GDH2 or GLUDP1, are both mitochondrial matrix enzymes belonging to the Glu/Leu/Phe/Val dehydrogenases family. Exisiting as homohexamers, GLUD1 catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia while GLUD2 is involved in the recycling of glutamate during neurotransmission. GLUD1 is critical for regulating amino acid induced insulin secretion and is allosterically activated by ADP and inhibited by GTP and ATP. Mutations in the gene encoding GLUD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLUD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. GLUD2 is expressed in testis and retina, with lower levels found in brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 55kDa
NCBI Official Full Name
glutamate dehydrogenase 2, mitochondrial
NCBI Official Synonym Full Names
glutamate dehydrogenase 2
NCBI Official Symbol
GLUD2
NCBI Official Synonym Symbols
GDH2; GLUDP1
NCBI Protein Information
glutamate dehydrogenase 2, mitochondrial
UniProt Protein Name
Glutamate dehydrogenase 2, mitochondrial
UniProt Gene Name
GLUD2
UniProt Synonym Gene Names
GLUDP1; GDH 2
UniProt Entry Name
DHE4_HUMAN
NCBI Description
The protein encoded by this gene is localized to the mitochondrion and acts as a homohexamer to recycle glutamate during neurotransmission. The encoded enzyme catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate. This gene is intronless.[provided by RefSeq, Jan 2010]
Uniprot Description
Function: Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
Catalytic activity: L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Subunit structure: Homohexamer
By similarity.
Subcellular location: Mitochondrion matrix Ref.7.
Tissue specificity: Expressed in retina, testis and, at a lower level, brain.
Post-translational modification: Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.
Sequence similarities: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
Research Articles on GLUD2
Similar Products
Product Notes
The GLUD2 glud2 (Catalog #AAA3011620) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLUD2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GLUD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:10-1:100. Researchers should empirically determine the suitability of the GLUD2 glud2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLUD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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