Rabbit GIPR / GIP Receptor Polyclonal Antibody | anti-GIPR antibody

Rabbit Polyclonal to Human GIPR / GIP Receptor

Cat. Num. AAA247959

• Gene Names: GIPR; PGQTL2
• Reactivity: Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit, Sheep
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: GIPR / GIP Receptor; Polyclonal Antibody; Rabbit Polyclonal to Human GIPR / GIP Receptor; Anti-GIPR / GIP Receptor Antibody (Cytoplasmic Domain) IHC-plus; GIPR; GIP receptor; PGQTL2; GIP-R; Human GIPR; GIP Receptor; anti-GIPR antibody

• Host: Rabbit
• Reactivity: Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit, Sheep
• Clonality: Polyclonal
• Specificity: Human GIPR. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GHRHR (100%), GCGR (61%), GLP2R (61%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 466

• Applicable Applications for anti-GIPR antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (32 ug/ml)
• Target: GIPR/GIP Receptor
• Immunogen Type: Synthetic peptide
• Immunogen Species: GIPR/GIP Receptor antibody was rasied against Human
• Immunogen Description: GIPR / GIP Receptor antibody was raised against synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human GIPR. Percent identity with opther species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Sheep, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit (94%); Opossum (83%).
• Epitope: Cytoplasmic Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-GIPR / GIP Receptor antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-GIPR antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-GIPR antibody

GIPR, or Gastric Inhibitory Polypeptide Receptor, mediates GIP-induced secretion of insulin by pancreatic islet beta cells after a meal. GIP is a gastrointestinal peptide hormone of 42 aa that is released from duodenal endocrine K cells after absorption of glucose or fat. Stimulation of the GIPR on pancreatic cells activates adenylyl cyclase and mitogen-activated protein kinase, resulting in increased insulin secretion.

Product Categories/Family for anti-GIPR antibody

Family: GPCR; Subfamily: Gastric inhibitory polypeptide

NCBI and Uniprot Product Information

• NCBI GI #: 4503999
• NCBI GeneID: 2696
• NCBI Accession #: NP_000155.1
• NCBI GenBank Nucleotide #: NM_000164.2
• UniProt Accession #: P48546; Q14401; Q16400; Q52M04; Q9UPI1; B7WP14; B7ZKQ0
• NCBI Official Full Name: gastric inhibitory polypeptide receptor
• NCBI Official Synonym Full Names: gastric inhibitory polypeptide receptor
• NCBI Official Symbol: GIPR
• NCBI Official Synonym Symbols: PGQTL2
• NCBI Protein Information: gastric inhibitory polypeptide receptor; GIP-R; glucose-dependent insulinotropic polypeptide receptor
• UniProt Protein Name: Gastric inhibitory polypeptide receptor
• Protein Family: Gastric inhibitory polypeptide receptor
• UniProt Gene Name: GIPR
• UniProt Synonym Gene Names: GIP-R
• UniProt Entry Name: GIPR_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor for gastric inhibitory polypeptide (GIP), which was originally identified as an activity in gut extracts that inhibited gastric acid secretion and gastrin release, but subsequently was demonstrated to stimulate insulin release in the presence of elevated glucose. Mice lacking this gene exhibit higher blood glucose levels with impaired initial insulin response after oral glucose load. Defect in this gene thus may contribute to the pathogenesis of diabetes. [provided by RefSeq, Oct 2011]

Uniprot Description

GIPR: This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 2; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: plasma membrane; integral to membrane

Molecular Function: gastric inhibitory peptide receptor activity; transmembrane receptor activity; peptide hormone binding

Biological Process: generation of precursor metabolites and energy; elevation of cytosolic calcium ion concentration; desensitization of G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; adenylate cyclase activation; response to glucose stimulus; positive regulation of insulin secretion; response to axon injury; response to calcium ion; endocrine pancreas development; response to nutrient