Centromere Protein J Recombinant Protein | CENPJ recombinant protein

Recombinant Centromere Protein J (CENPJ)

Cat. Num. AAA2033087

• Gene Names: CENPJ; LAP; CPAP; LIP1; BM032; MCPH6; SASS4; SCKL4; Sas-4; CENP-J
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Centromere Protein J; Recombinant Centromere Protein J (CENPJ); LAP; LIP1; BM032; CPAP; MCPH6; Microcephaly; Primary Autosomal Recessive 6; Centrosomal P4.1-associated protein; LAG-3-associated protein; LYST-interacting protein 1; CENPJ recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MFLMPTSSEL NSGQNFLTQW MTNPSRAGVI LNRGFPILEA DKEKRAAVDI STSFPIKGTH FSDSFSFINE EDSLLEEQKL ESNNPYKPQS DKSETHTAFP CIKKGPQVAA CHSAPGHQEE NKNDFIPDLA SEFKEGAYKD PLFKKLEQLK EVQQKKQEQL KRQQLEQLQR LMEEQEKLLTMVSGQCTLPG LSLLPDDQSQ KHRSPGN
• Sequence Length: 1338

• Applicable Applications for CENPJ recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 27.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Asn207
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 130980075
• NCBI GeneID: 55835
• NCBI Accession #: NP_060921.3
• NCBI GenBank Nucleotide #: NM_018451.4
• Molecular Weight: 124,244 Da
• NCBI Official Full Name: centromere protein J
• NCBI Official Synonym Full Names: centromere protein J
• NCBI Official Symbol: CENPJ
• NCBI Official Synonym Symbols: LAP; CPAP; LIP1; BM032; MCPH6; SASS4; SCKL4; Sas-4; CENP-J
• NCBI Protein Information: centromere protein J
• UniProt Protein Name: Centromere protein J
• Protein Family: Centromere protein
• UniProt Gene Name: CENPJ
• UniProt Synonym Gene Names: CPAP; LAP; LIP1; CENP-J
• UniProt Entry Name: CENPJ_HUMAN

NCBI Description

This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

CENPJ: Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Defects in CENPJ are the cause of microcephaly primary type 6 (MCPH6). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. Defects in CENPJ are the cause of Seckel syndrome type 4 (SCKL4). SCKL4 is a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation. Belongs to the TCP10 family.

Protein type: Cell cycle regulation; Microtubule-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 13q12.12

Cellular Component: centriole; centrosome; cytosol; gamma-tubulin small complex; microtubule

Molecular Function: protein binding; protein domain specific binding; protein kinase binding; tubulin binding

Biological Process: cell division; centriole replication; G2/M transition of mitotic cell cycle; microtubule nucleation; microtubule polymerization; mitotic cell cycle; organelle organization and biogenesis; regulation of centriole replication

Disease: Microcephaly 6, Primary, Autosomal Recessive; Seckel Syndrome 4