Rabbit anti-Human GIPR Polyclonal Antibody | anti-GIPR antibody

GIPR, ID (GIPR, Gastric inhibitory polypeptide receptor, Glucose-dependent insulinotropic polypeptide receptor) (AP)

Cat. Num. AAA6302314

• Gene Names: GIPR; PGQTL2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GIPR; Polyclonal Antibody; ID (GIPR; Gastric inhibitory polypeptide receptor; Glucose-dependent insulinotropic polypeptide receptor) (AP); Glucose-dependent insulinotropic polypeptide receptor; anti-GIPR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GIPR antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GIPR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113~142 amino acids from the Center region of human GIPR.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GIPR Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). GIPR (arrow) was detected using the purified Pab.)

Related Product Information for anti-GIPR antibody

GIPR also called glucose-dependent insulinotropic polypeptide, is a 42-amino acid polypeptide synthesized by K cells of the duodenum and small intestine. This protein was originally identified as an activity in gut extracts that inhibited gastric acid secretion and gastrin release, but subsequently was demonstrated to stimulate insulin release potently in the presence of elevated glucose. The insulinotropic effect on pancreatic islet beta-cells was then recognized to be the principal physiologic action of GIP. Together with glucagon-like peptide-1, GIP is largely responsible for the secretion of insulin after eating. The protein is involved in several other facets of the anabolic response.

Product Categories/Family for anti-GIPR antibody

Antibodies; Insulin

References

Herbach, N. Am. J. Physiol. Renal Physiol. 296 (4), F819-F829 (2009). Rudovich, N., Kaiser, S. Regul. Pept. 142 (3), 138-145 (2007). Nitz, I., Fisher, E. Mol Nutr Food Res 51 (8), 1046-1052 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4503999
• NCBI GeneID: 2696
• NCBI Accession #: NP_000155.1
• NCBI GenBank Nucleotide #: NM_000164.2
• UniProt Accession #: P48546; Q14401; Q16400; Q52M04; Q9UPI1
• Molecular Weight: 53,157 Da
• NCBI Official Full Name: gastric inhibitory polypeptide receptor
• NCBI Official Synonym Full Names: gastric inhibitory polypeptide receptor
• NCBI Official Symbol: GIPR
• NCBI Official Synonym Symbols: PGQTL2
• NCBI Protein Information: gastric inhibitory polypeptide receptor; GIP-R; glucose-dependent insulinotropic polypeptide receptor
• UniProt Protein Name: Gastric inhibitory polypeptide receptor
• Protein Family: Gastric inhibitory polypeptide receptor
• UniProt Gene Name: GIPR
• UniProt Synonym Gene Names: GIP-R
• UniProt Entry Name: GIPR_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor for gastric inhibitory polypeptide (GIP), which was originally identified as an activity in gut extracts that inhibited gastric acid secretion and gastrin release, but subsequently was demonstrated to stimulate insulin release in the presence of elevated glucose. Mice lacking this gene exhibit higher blood glucose levels with impaired initial insulin response after oral glucose load. Defect in this gene thus may contribute to the pathogenesis of diabetes. [provided by RefSeq, Oct 2011]

Uniprot Description

GIPR: This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: gastric inhibitory peptide receptor activity; transmembrane receptor activity; peptide hormone binding

Biological Process: elevation of cytosolic calcium ion concentration; desensitization of G-protein coupled receptor protein signaling pathway; generation of precursor metabolites and energy; cell surface receptor linked signal transduction; adenylate cyclase activation; response to glucose stimulus; positive regulation of insulin secretion; response to axon injury; response to calcium ion; endocrine pancreas development; response to nutrient