HIV-p51/RT-p51 (group M, subtype B (isolate HXB2) Gag-Pol polyprotein Protein Recombinant Protein | Gag-Pol recombinant protein

Recombinant HIV-p51/RT-p51 (group M, subtype B (isolate HXB2) Gag-Pol polyprotein Protein (His Tag)

Cat. Num. AAA2569543

• Purity: >90% as determined by SDS-PAGE
• Synonyms: HIV-p51/RT-p51 (group M; subtype B (isolate HXB2) Gag-Pol polyprotein Protein; Recombinant HIV-p51/RT-p51 (group M; subtype B (isolate HXB2) Gag-Pol polyprotein Protein (His Tag); Gag-Pol Protein; HIV; Gag-Pol recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50 mM Tris, 250 mM Nacl, 1 mM EDTA, 50% glycerol, pH7.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
• Sequence: Pro588-Phe1027

• Species: HIV
• Tag: N-His
• Endotoxin: Please contact us for more information.
• Protein Construction: A DNA sequence encoding the p51 (Reverse Transcriptase) of human immunodeficiency virus 1 gag-pol (NP_057849.4)(Pro588-Phe1027) was expressed with a polyhistidine tag at the N-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Product Categories/Family for Gag-Pol recombinant protein

Virus related Protein

NCBI and Uniprot Product Information

• NCBI GI #: 28872819
• NCBI GeneID: 155348
• NCBI Accession #: NP_057849.4
• NCBI GenBank Nucleotide #: NC_001802.1
• UniProt Accession #: P04585; O09777; Q9WJC5
• Molecular Weight: 24kDa (mature); 55kDa; 41kDa (precursors)
• NCBI Official Full Name: Gag-Pol
• NCBI Official Symbol: gag-pol
• NCBI Protein Information: Gag-Pol; Gag-Pol polyprotein; Gag-Pol Transframe peptide; aspartic peptidase; p66 subunit; integrase; reverse transcriptase p51 subunit
• UniProt Protein Name: Gag-Pol polyprotein
• Protein Family: Gag-Pol polyprotein
• UniProt Gene Name: P04585.
• UniProt Synonym Gene Names: MA; CA; NC; TF; p6*; IN
• UniProt Entry Name: POL_HV1H2

Uniprot Description

Gag-Pol polyprotein: Mediates, with Gag polyrotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi). Gag-Pol polyprotein may regulate its own translation, by the binding genomic RNA in the 5'-UTR. At low concentration, the polyprotein would promote translation, whereas at high concentration, the polyprotein would encapsidate genomic RNA and then shutt off translation.Matrix protein p17: Targets the polyprotein to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus (). Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA ().