Rabbit GHRHR Polyclonal Antibody | anti-GHRHR antibody

GHRHR Polyclonal Antibody

Cat. Num. AAA3005875

• Gene Names: GHRHR; GRFR; GHRFR; IGHD4; IGHD1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GHRHR; Polyclonal Antibody; GHRHR Polyclonal Antibody; Growth hormone-releasing hormone receptor; GHRH receptor; Growth hormone-releasing factor receptor; GRF receptor; GRFR; GHRH-R; anti-GHRHR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GHRHR polyclonal antibody detects endogenous levels of GHRHR protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 423

• Applicable Applications for anti-GHRHR antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 378-423 of Human GHRHR.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GHRHR polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse liver tissue lysateLane3:Rat liver tissue lysate)

Related Product Information for anti-GHRHR antibody

GHRH-R (growth hormone-releasing hormone receptor) is a seven transmembrane domain protein that localizes to the somatotroph of the pituitary. GHRH-R plays an important role in growth and acts as a high-affinity receptor for GHRH. Binding of GHRH leads to the coupling of GHRH-R to G-protein which stimulates increased adenylyl cyclase activity and the accumulation of cAMP leading to the synthesis and release of growth hormone and somatotroph proliferation. In addition, this signalling pathway may have direct action in fetal/placental development, reproduction and immune function. GHRH and GHRH-R may also play a role in the regulation of non-rapid eye movement sleep (NREMS). The expression of GHRH-R is dependent on the presence of the POU domain factor Pit-1. Mutations in the gene encoding this protein can result in isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, and anterior pituitary hypoplasia (APH).

NCBI and Uniprot Product Information

• NCBI GI #: 58530851
• NCBI GeneID: 2692
• NCBI Accession #: NP_000814.2
• NCBI GenBank Nucleotide #: NP_000814.2
• UniProt Accession #: Q02643
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: growth hormone-releasing hormone receptor
• NCBI Official Synonym Full Names: growth hormone releasing hormone receptor
• NCBI Official Symbol: GHRHR
• NCBI Official Synonym Symbols: GRFR; GHRFR; IGHD4; IGHD1B
• NCBI Protein Information: growth hormone-releasing hormone receptor
• UniProt Protein Name: Growth hormone-releasing hormone receptor
• Protein Family: Growth hormone-releasing hormone receptor
• UniProt Gene Name: GHRHR
• UniProt Synonym Gene Names: GHRH receptor; GRF receptor; GRFR
• UniProt Entry Name: GHRHR_HUMAN

NCBI Description

This gene encodes a receptor for growth hormone-releasing hormone. Binding of this hormone to the receptor leads to synthesis and release of growth hormone. Mutations in this gene have been associated with isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, a disorder characterized by short stature. [provided by RefSeq, Jun 2010]

Uniprot Description

GHRHR: Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Belongs to the G-protein coupled receptor 2 family.

Protein type: GPCR, family 2; Membrane protein, integral; Cell development/differentiation; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear outer membrane; cell surface; nuclear matrix; cytoplasm; integral to membrane; plasma membrane; nuclear inner membrane; secretory granule

Molecular Function: G-protein coupled receptor activity; protein binding; peptide hormone binding; growth factor binding; growth hormone-releasing hormone receptor activity

Biological Process: lactation; positive regulation of insulin-like growth factor receptor signaling pathway; response to glucocorticoid stimulus; regulation of protein metabolic process; cell maturation; adenylate cyclase activation; positive regulation of multicellular organism growth; determination of adult life span; reproductive process in a multicellular organism; G-protein signaling, adenylate cyclase activating pathway; response to insulin stimulus; positive regulation of growth hormone secretion; water homeostasis; growth hormone secretion; cAMP-mediated signaling; cellular response to insulin stimulus; response to estrogen stimulus; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; somatotropin secreting cell development; hormone metabolic process; regulation of steroid hormone receptor signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Ib