Testing Data
Rabbit Calpain-15 Polyclonal Antibody | anti-CAPN15 antibody
Anti-Calpain-15 (SOLH, Small Optic Lobe Protein), Domain-I, Large Subunit Antibody
Gene Names
CAPN15; SOLH
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Calpain-15; Polyclonal Antibody; Anti-Calpain-15 (SOLH; Small Optic Lobe Protein); Domain-I; Large Subunit Antibody; anti-CAPN15 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody binds to Calpain 15, but does not cross react with the other calpain family members (calpain 1, Calpain 2, ncl 2, ncl 3, etc.) and recognizes latent but not the aminotruncated (active) Calpain 15.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
1086
Applicable Applications for anti-CAPN15 antibody
Western Blot (WB)
Application Notes
Calpain-15 is an intracellular, calcium-dependent cysteine protease. Calpain-15 was first described as the human homologue of SOL, the small optic lobe protein, a drosophila protein. The calpain-15 sequence is very different from the classical calpains, and has a much longer aminoterminal end. In fact, about the only similarity between SOLH and the classical calpains is the catalytic residues, CHN, which are all conserved in calpain-15. Sequence alignments place Calpain-15 closest to calpain-7. The calpain-15 sequence is a whopping 1086 amino acids long, predicting a protein of 117.313 kD, much larger that other calpain family members (almost all of the difference is in the amino end). RP1Calpain-15 recognizes latent but not the aminotruncated (active) Calpain-15. RP1Calpain-15 Anti Calpain-15 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 115 kD (minor band), and a series of further cleaved forms. RP1Calpain-15 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Not all cell types produce Calpain-15; since it is a cellular protein, cell lysate works well for Western blots. Rat, mouse and human Calpain-15 are detected with RP1Calpain-15. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.
Immunogen
Synthetic peptide based on the aminoterminal end of domain-I in the large subunit of Human Calpain 15
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-CAPN15 antibody
Description: The antibody is a Rabbit antibody to the cysteine protease, Calpain-15, made with a synthetic peptide based on the aminoterminal end of domain-I in the large subunit, using the human sequence. The antibody binds to Calpain-15, but does not cross react with the other calpain family members (calpain-1, Calpain-2, ncl-2, ncl-3, etc.). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Relevance: Calpain 15 is an intracellular, calcium dependent cysteine protease. Calpain 15 was first described as the human homologue of SOL, the small optic lobe protein, in drosophila. The Calpain 15 sequence is very different from the classical calpains, and has an extended aminoterminal domain. In fact, about the only similarity between this and the classical calpains is the catalytic residues, CHN, which are all conserved in Calpain 15. Sequence alignments place Calpain 15 closest to Calpain 7. The Calpain 15 sequence is 1086 amino acids long, predicting a protein of 117kD, much larger than other calpain family members (almost all of the difference is in the amino end).
Relevance: Calpain 15 is an intracellular, calcium dependent cysteine protease. Calpain 15 was first described as the human homologue of SOL, the small optic lobe protein, in drosophila. The Calpain 15 sequence is very different from the classical calpains, and has an extended aminoterminal domain. In fact, about the only similarity between this and the classical calpains is the catalytic residues, CHN, which are all conserved in Calpain 15. Sequence alignments place Calpain 15 closest to Calpain 7. The Calpain 15 sequence is 1086 amino acids long, predicting a protein of 117kD, much larger than other calpain family members (almost all of the difference is in the amino end).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
calpain-15
NCBI Official Synonym Full Names
calpain 15
NCBI Official Symbol
CAPN15
NCBI Official Synonym Symbols
SOLH
NCBI Protein Information
calpain-15
UniProt Protein Name
Calpain-15
Protein Family
UniProt Gene Name
CAPN15
UniProt Synonym Gene Names
SOLH
UniProt Entry Name
CAN15_HUMAN
NCBI Description
This gene encodes a protein containing zinc-finger-like repeats and a calpain-like protease domain. The encoded protein may function as a transcription factor, RNA-binding protein, or in protein-protein interactions during visual system development. [provided by RefSeq, Jul 2008]
Similar Products
Product Notes
The CAPN15 capn15 (Catalog #AAA4157434) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Calpain-15 (SOLH, Small Optic Lobe Protein), Domain-I, Large Subunit Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calpain-15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Calpain-15 is an intracellular, calcium-dependent cysteine protease. Calpain-15 was first described as the human homologue of SOL, the small optic lobe protein, a drosophila protein. The calpain-15 sequence is very different from the classical calpains, and has a much longer aminoterminal end. In fact, about the only similarity between SOLH and the classical calpains is the catalytic residues, CHN, which are all conserved in calpain-15. Sequence alignments place Calpain-15 closest to calpain-7. The calpain-15 sequence is a whopping 1086 amino acids long, predicting a protein of 117.313 kD, much larger that other calpain family members (almost all of the difference is in the amino end). RP1Calpain-15 recognizes latent but not the aminotruncated (active) Calpain-15. RP1Calpain-15 Anti Calpain-15 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 115 kD (minor band), and a series of further cleaved forms. RP1Calpain-15 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Not all cell types produce Calpain-15; since it is a cellular protein, cell lysate works well for Western blots. Rat, mouse and human Calpain-15 are detected with RP1Calpain-15. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Researchers should empirically determine the suitability of the CAPN15 capn15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calpain-15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
