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Western Blot (WB) (A4GALT Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the A4GALT antibody detected the A4GALT protein (arrow).)

Rabbit anti-Human A4GALT Polyclonal Antibody | anti-A4GALT antibody

A4GALT, NT (A4GALT, A14GALT, A4GALT1, Lactosylceramide 4-alpha-galactosyltransferase, Alpha-1,4-N-acetylglucosaminyltransferase, Alpha-1,4-galactosyltransferase, Alpha4Gal-T1, CD77 synthase, Globotriaosylceramide synthase, P1/Pk synthase, UDP-galactose:be

Gene Names
A4GALT; P1; PK; Gb3S; P(k); P1PK; A14GALT; A4GALT1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
A4GALT; Polyclonal Antibody; NT (A4GALT; A14GALT; A4GALT1; Lactosylceramide 4-alpha-galactosyltransferase; Alpha-1; 4-N-acetylglucosaminyltransferase; 4-galactosyltransferase; Alpha4Gal-T1; CD77 synthase; Globotriaosylceramide synthase; P1/Pk synthase; UDP-galactose:be; UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase; anti-A4GALT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-A4GALT antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
A4GALT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human A4GALT.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(A4GALT Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the A4GALT antibody detected the A4GALT protein (arrow).)

Western Blot (WB) (A4GALT Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the A4GALT antibody detected the A4GALT protein (arrow).)
Related Product Information for anti-A4GALT antibody
The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor.
Product Categories/Family for anti-A4GALT antibody
References
Zumbrun, S.D., et al. Infect. Immun. 78(11):4488-4499(2010) Shin, I.S., et al. BMB Rep 42(5):310-314(2009) Lund, N., et al. Blood 113(20):4980-4991(2009) Okuda, T., et al. Glycobiology 18(12):1028-1035(2008) Hellberg, A., et al. BMC Genet. 6, 49 (2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,499 Da
NCBI Official Full Name
lactosylceramide 4-alpha-galactosyltransferase
NCBI Official Synonym Full Names
alpha 1,4-galactosyltransferase
NCBI Official Symbol
A4GALT
NCBI Official Synonym Symbols
P1; PK; Gb3S; P(k); P1PK; A14GALT; A4GALT1
NCBI Protein Information
lactosylceramide 4-alpha-galactosyltransferase; GB3 synthase; alpha4Gal-T1; CD77 synthase; P1/Pk synthase; P(k) antigen synthase; P blood group (P one antigen); globotriaosylceramide synthase; alpha-1,4-N-acetylglucosaminyltransferase; UDP-galactose:beta-
UniProt Protein Name
Lactosylceramide 4-alpha-galactosyltransferase
UniProt Gene Name
A4GALT
UniProt Synonym Gene Names
A14GALT; A4GALT1; Gb3 synthase
UniProt Entry Name
A4GAT_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

A4GALT: Necessary for the biosynthesis of the Pk antigen of blood histogroup P. Catalyzes the transfer of galactose to lactosylceramide and galactosylceramide. Necessary for the synthesis of the receptor for bacterial verotoxins. Belongs to the glycosyltransferase 32 family.

Protein type: EC 2.4.1.228; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: membrane; integral to Golgi membrane

Molecular Function: toxin binding; galactosyltransferase activity; lactosylceramide 4-alpha-galactosyltransferase activity

Biological Process: globoside biosynthetic process; glycosphingolipid biosynthetic process; plasma membrane organization and biogenesis; protein amino acid glycosylation

Disease: Blood Group, P1pk System

Research Articles on A4GALT

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Product Notes

The A4GALT a4galt (Catalog #AAA6270298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The A4GALT, NT (A4GALT, A14GALT, A4GALT1, Lactosylceramide 4-alpha-galactosyltransferase, Alpha-1,4-N-acetylglucosaminyltransferase, Alpha-1,4-galactosyltransferase, Alpha4Gal-T1, CD77 synthase, Globotriaosylceramide synthase, P1/Pk synthase, UDP-galactose:be reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's A4GALT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the A4GALT a4galt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "A4GALT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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