Western Blot (WB)
(GFRA4 Antibody (Center) western blot analysis in HL-60,ZR-75-1,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the GFRA4 antibody detected the GFRA4 protein (arrow).)
Rabbit anti-Human GFRA4 Polyclonal Antibody | anti-GFRA4 antibody
GFRA4, ID (GFRA4, GDNF family receptor alpha-4, Persephin receptor) (FITC)
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GFRA4; Polyclonal Antibody; ID (GFRA4; GDNF family receptor alpha-4; Persephin receptor) (FITC); Persephin receptor; anti-GFRA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-GFRA4 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
GFRA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-184 amino acids from the Central region of human GFRA4.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(GFRA4 Antibody (Center) western blot analysis in HL-60,ZR-75-1,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the GFRA4 antibody detected the GFRA4 protein (arrow).)
Western Blot (WB)
(GFRA4 Antibody (Center) western blot analysis in HL-60,ZR-75-1,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the GFRA4 antibody detected the GFRA4 protein (arrow).)
Related Product Information for anti-GFRA4 antibody
The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for RET-associated diseases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Product Categories/Family for anti-GFRA4 antibody
References
Yang, J., et al. FEBS Lett. 569 (1-3), 267-271 (2004): Borrego, S., et al. J. Med. Genet. 40 (3), E18 (2003): Deloukas, P., et al. Nature 414(6866):865-871(2001). Zhou, B., et al. Pediatr. Neurol. 25(2):156-161(2001). Lindahl, M., et al. J. Biol. Chem. 276(12):9344-9351(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,216 Da
NCBI Official Full Name
GDNF family receptor alpha-4 isoform b
NCBI Official Synonym Full Names
GDNF family receptor alpha 4
NCBI Official Symbol
GFRA4
NCBI Protein Information
GDNF family receptor alpha-4; GDNF receptor alpha-4; GDNFR-alpha-4; GFR receptor alpha 4; GFR-alpha-4; persephin receptor
UniProt Protein Name
GDNF family receptor alpha-4
Protein Family
UniProt Gene Name
GFRA4
UniProt Synonym Gene Names
GDNF receptor alpha-4; GDNFR-alpha-4; GFR-alpha-4
UniProt Entry Name
GFRA4_HUMAN
NCBI Description
The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for RET-associated diseases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Receptor for persephin. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. May be important in C-cell development and, in the postnatal development of the adrenal medulla.
Subcellular location: Isoform GFRalpha4a: Cell membrane; Lipid-anchor › GPI-anchor Ref.1. Isoform GFRalpha4b: Cell membrane; Lipid-anchor › GPI-anchor Ref.1. Isoform GFRalpha4c: Secreted.
Tissue specificity: Predominantly expressed in the adult thyroid gland. Low levels also found in fetal adrenal and thyroid glands.
Sequence similarities: Belongs to the GDNFR family.
Research Articles on GFRA4
Similar Products
Product Notes
The GFRA4 gfra4 (Catalog #AAA6302009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GFRA4, ID (GFRA4, GDNF family receptor alpha-4, Persephin receptor) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GFRA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GFRA4 gfra4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFRA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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