Rabbit anti-Human ELF3 Polyclonal Antibody | anti-ELF3 antibody

ELF3, CT (ELF3, ERT, ESX, JEN, ETS-related transcription factor Elf-3, E74-like factor 3, Epithelial-restricted with serine box, Epithelium-restricted Ets protein ESX, Epithelium-specific Ets transcription factor 1)

Cat. Num. AAA648631

• Gene Names: ELF3; ERT; ESX; EPR-1; ESE-1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ELF3; Polyclonal Antibody; CT (ELF3; ERT; ESX; JEN; ETS-related transcription factor Elf-3; E74-like factor 3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1); Anti -ELF3; anti-ELF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ELF3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: ELF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328~358 amino acids from the C-terminal region of human ELF3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ELF3 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). ELF3 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ELF3 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 167235023
• NCBI GeneID: 1999
• NCBI Accession #: NP_004424.3
• NCBI GenBank Nucleotide #: NM_004433.4
• UniProt Accession #: P78545; Q6IAP8; Q7RU03; Q7Z3X2; Q8NFG2; Q99718; B2R5Q6
• Molecular Weight: 41,454 Da
• NCBI Official Full Name: ETS-related transcription factor Elf-3
• NCBI Official Synonym Full Names: E74-like factor 3 (ets domain transcription factor, epithelial-specific )
• NCBI Official Symbol: ELF3
• NCBI Official Synonym Symbols: ERT; ESX; EPR-1; ESE-1
• NCBI Protein Information: ETS-related transcription factor Elf-3; epithelial-restricted with serine box; epithelium-restricted Ets protein ESX; epithelium-specific Ets transcription factor 1; E74-like factor 3 (ets domain transcription factor); ets domain transcription factor, serine box (epithelial-specific); E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific)
• UniProt Protein Name: ETS-related transcription factor Elf-3
• Protein Family: ELF3-like protein
• UniProt Gene Name: ELF3
• UniProt Synonym Gene Names: ERT; ESX; JEN; ESE-1
• UniProt Entry Name: ELF3_HUMAN

Uniprot Description

Function: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development

By similarity. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.15 Ref.16 Ref.17 Ref.18 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30

Subunit structure: Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3. Ref.6 Ref.22 Ref.25 Ref.30

Subcellular location: Cytoplasm. Nucleus. Note: Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism. Ref.6 Ref.26 Ref.30

Tissue specificity: Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin. Ref.1 Ref.2 Ref.3 Ref.4

Induction: Transcriptionally regulated by ERBB2 receptor signaling in breast cancer epithelial cells. Up-regulated by phorbol 12-myristate 13-acetate (PMA) in bronchial epithelial cells. By retinoic acid in MCF-7 mammary epithelial cells (at protein level). Ref.19 Ref.23 Ref.29

Domain: the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.31

Sequence similarities: Belongs to the ETS family.Contains 1 ETS DNA-binding domain.Contains 1 PNT (pointed) domain.