Bovine S100-A12 ELISA Kit | S100A12 elisa kit

Bovine Protein S100-A12 ELISA Kit

Cat. Num. AAA2886444

• Gene Names: S100A12; CAGC; CAAF1; CO-AG; EN-RAGE
• Reactivity: Bovine
• Synonyms: S100-A12; Bovine Protein S100-A12 ELISA Kit; Protein S100-A12; Calcium-binding protein in amniotic fluid 1; CAAF1; Calgranulin-C; CAGC; Cornea-associated antigen; CO-AG; Extracellular newly identified RAGE-binding protein; EN-RAGE; S100A12 elisa kit

• Reactivity: Bovine
• Specificity: Recombinant and natural Bovine Protein S100-A12
• Sequence Length: 92

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: < 0.055 ng/mL
• Intra-assay Precision: <=5.6%
• Inter-assay Precision: <=9.3%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A12 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27807183
• NCBI GeneID: 282467
• NCBI Accession #: NP_777076.1
• NCBI GenBank Nucleotide #: NM_174651.3
• UniProt Accession #: P79105; Q3T070; Q9TR16
• Molecular Weight: 10,685 Da
• NCBI Official Full Name: protein S100-A12
• NCBI Official Symbol: S100A12
• NCBI Official Synonym Symbols: CAGC; CAAF1; CO-AG; EN-RAGE
• NCBI Protein Information: protein S100-A12
• UniProt Protein Name: Protein S100-A12
• Protein Family: Protein
• UniProt Gene Name: S100A12
• UniProt Synonym Gene Names: CAAF1; CAAF1; CAGC; CO-AG; EN-RAGE
• UniProt Entry Name: S10AC_BOVIN

Uniprot Description

S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn2+ from their active sites ().