Testing Data
(Anti-EGFP at 1/1000 ~ 1/5000 dilution)
Rabbit EGFP Polyclonal Antibody | anti-EGFP antibody
EGFP polyclonal antibody
Reactivity
Recombinant EGFP
Applications
Western Blot
Purity
affinity purified by Protein A
Synonyms
EGFP; Polyclonal Antibody; EGFP polyclonal antibody; Enhanced Green Fluorecent protein; rEGFP; Recombinant EGFP; Enhanced Green Fluorecent protein.; anti-EGFP antibody
Host
Rabbit
Reactivity
Recombinant EGFP
Clonality
Polyclonal
Specificity
EGFP polyclonal Antibody detects endogenous levels of EGFP protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-EGFP antibody
Western Blot (WB)
Application Notes
WB: 1:1000-10000
Immunogen
Recombinant EGFP protin:
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Testing Data
(Anti-EGFP at 1/1000 ~ 1/5000 dilution)
Testing Data
(Anti-EGFP at 1/1000 ~ 1/5000 dilution)
Testing Data
(Anti-EGFP at 1/1000 ~ 1/5000 dilution)
Testing Data
(Anti-EGFP at 1/1000 ~ 1/5000 dilution)
Testing Data
(Anti-EGFP at 1/1000 dilution)
Testing Data
(Anti-EGFP at 1/1000 dilution)
Related Product Information for anti-EGFP antibody
Enhanced green fluorescence protein (EGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). EGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP fluorescence is stable under fixation conditions and suitable for a variety of applications. EGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize EGFP-tagged proteins within living cells without the need for chemical staining. Other applications of EGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. EGFP technnology has considerably contributed to a greater understanding of cellular physiology.Description: EGFP produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27kDa. EGFP is purified by proprietary chromatographic techniques
NCBI and Uniprot Product Information
Uniprot Description
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
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Product Notes
The EGFP gfp (Catalog #AAA3008729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EGFP polyclonal antibody reacts with Recombinant EGFP and may cross-react with other species as described in the data sheet. AAA Biotech's EGFP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-10000. Researchers should empirically determine the suitability of the EGFP gfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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