Western Blot (WB)
(Western blot analysis of GFP usign recombinant RFP (dsRed Lane 1 & 2), YFP (Lane 3 & 4) and EGFP (Lane 5 & 6).)
Rabbit GFP Polyclonal Antibody | anti-GFP antibody
GFP Antibody
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Chromatography
Synonyms
GFP; Polyclonal Antibody; GFP Antibody; Rabbit Polyclonal GFP Antibody; Polyclonal GFP antibody; anti-GFP antibody; Green Fluorescent Protein antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer, pH 7.2, containing 30% Glycerol, 0.5% BSA, 0.01% Thimerosal
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-GFP antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 10-20 ug/ml
WB: 0.5-4 ug/ml
WB: 0.5-4 ug/ml
Immunogen
GFP antibody was raised in Rabbit using recombinant EGFP as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of GFP usign recombinant RFP (dsRed Lane 1 & 2), YFP (Lane 3 & 4) and EGFP (Lane 5 & 6).)
Western Blot (WB)
(Western blot analysis of GFP usign recombinant RFP (dsRed Lane 1 & 2), YFP (Lane 3 & 4) and EGFP (Lane 5 & 6).)
Related Product Information for anti-GFP antibody
Green fluorescent protein is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
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Product Notes
The GFP (Catalog #AAA5311570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). IP: 10-20 ug/ml WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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