Western Blot (WB)
(Western blot analysis of rat brain microsome extract using MBS610628 at 0.5ug/ml.)
Rabbit mGluR2,3 Polyclonal Antibody
mGluR2,3 (Metabotropic Glutamate Receptor)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
mGluR2; 3; Polyclonal Antibody; 3 (Metabotropic Glutamate Receptor); Anti -mGluR2; anti-mGluR2; 3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mGluR2 and mGluR3, MW
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%
Applicable Applications for anti-mGluR2,3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Immunoblot, Immunoprecipitation, and Immunohistochemistry (paraffin embedded sections).
Dilution: Immunoblot Analysis: 0.5-2ug/ml detects mGluR2/3 in 20ug of rat brain microsomal preparation. Note: Do not boil the microsomal preparation, heat to 37 degree C for 15-30 minutes. 20ug of rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M3884-76 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates mGluR2/3 from 400ug of rat brain microsomal preparation.
Immunohistochemistry: Detects mGluR 2/3 in paraffin embedded rat brain tissue sections.
Optimal dilution determined by the researcher.
Dilution: Immunoblot Analysis: 0.5-2ug/ml detects mGluR2/3 in 20ug of rat brain microsomal preparation. Note: Do not boil the microsomal preparation, heat to 37 degree C for 15-30 minutes. 20ug of rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M3884-76 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates mGluR2/3 from 400ug of rat brain microsomal preparation.
Immunohistochemistry: Detects mGluR 2/3 in paraffin embedded rat brain tissue sections.
Optimal dilution determined by the researcher.
Immunogen
Synthetic peptide (KQFVPTVCNGREVVDSTTSSL) corresponding to aa853-872 of human metabotropic glutamate receptor 2, with a lysine added to the N-terminus for conjugation (KLH conjugated).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of rat brain microsome extract using MBS610628 at 0.5ug/ml.)
Western Blot (WB)
(Western blot analysis of rat brain microsome extract using MBS610628 at 0.5ug/ml.)
Related Product Information for anti-mGluR2,3 antibody
Metabotropic glutamate receptors have been classified into three groups. In general, class I receptors (mGluR1 and mGluR5) mediate the excitation of neurons, whereas activation of class II (mGluR2 and mGluR3) and class III (mGluR4a, mGluR7a, mGluR7b and mGluR8) results in a depression of synaptic transmission. Class I mGluRs are localized in the postsynaptic membrane and are positively coupled to phospholipase C (PLC). Class II and class III mGluRs localized to the presynaptic membrane and are negatively coupled to adenylate cyclase. It has been speculated that mGluR2 and/or mGluR3 may play a role in the modulation of the mossy terminal function.
Product Categories/Family for anti-mGluR2,3 antibody
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Product Notes
The mGluR2,3 (Catalog #AAA610628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mGluR2,3 (Metabotropic Glutamate Receptor) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's mGluR2,3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Immunoblot, Immunoprecipitation, and Immunohistochemistry (paraffin embedded sections). Dilution: Immunoblot Analysis: 0.5-2ug/ml detects mGluR2/3 in 20ug of rat brain microsomal preparation. Note: Do not boil the microsomal preparation, heat to 37 degree C for 15-30 minutes. 20ug of rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M3884-76 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates mGluR2/3 from 400ug of rat brain microsomal preparation. Immunohistochemistry: Detects mGluR 2/3 in paraffin embedded rat brain tissue sections. Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the mGluR2,3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mGluR2,3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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